| HLA-DR expression is induced on keratinocytes in delayed hypersensitivity but not in allergen induced late-phase reactions. | |
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MedLine Citation:
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PMID: 7788573 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In view of increasing evidence suggesting an active immunoregulatory role of the skin keratinocytes and the observation that the differentiation of allergen specific T lymphocytes is critical in the development of allergy, we evaluated epidermal expression of HLA-DR antigen in skin reactions induced with an atopen (house dust mite) and with an non-atopic antigen (Hemocyanin). Two groups of patients with house dust mite (Dermatophagoides pteronyssinus [Der p]) allergy were compared, one group was skin tested with Der p, the other group was immunized and subsequently skin tested with Helix pomatia Hemocyanin (HPH). Biopsy specimens taken at 48 h after the HPH (n = 11) and Der p (n = 11) tests were analysed immunohistologically. Reactions in both groups were comparable in size. Immunohistological analysis showed domination by CD4+ lymphocytes. Expression of HLA-DR antigen by epidermal keratinocytes was observed in six out of 11 of the HPH induced reactions, but in none of the Der p induced reactions. Eosinophils were spotted only throughout the Der p induced reactions, showing a good correlation with the number of CD4 positive lymphocytes. The lack of HLA-DR expression by keratinocytes during the allergen-induced reaction, compared with the Hemocyanin induced reaction can be the result of a difference in cytokine profile of the lymphocytes dominating the dermal infiltrate. On the other hand evidence exists that defective HLA-DR expression by keratinocytes enhances antigen induced lymphocyte activation, and may thus contribute to the development of allergen-specific T-lymphocytes. |
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Authors:
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F R Weller; M C De Jong; M S Weller; K Heeres; J G De Monchy; H M Jansen |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology Volume: 25 ISSN: 0954-7894 ISO Abbreviation: Clin. Exp. Allergy Publication Date: 1995 Mar |
Date Detail:
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Created Date: 1995-07-24 Completed Date: 1995-07-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8906443 Medline TA: Clin Exp Allergy Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 252-9 Citation Subset: IM |
Affiliation:
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Department of Pulmonology, University of Amsterdam, the Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Allergens
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adverse effects Animals Antigens, Dermatophagoides CD4-Positive T-Lymphocytes / immunology Dermatitis, Allergic Contact / etiology, metabolism*, pathology Eosinophils / immunology Glycoproteins / adverse effects* HLA-DR Antigens / biosynthesis* Helix (Snails) / immunology Hemocyanin / adverse effects* Humans Hypersensitivity, Delayed / etiology, metabolism*, pathology Immunoenzyme Techniques Immunophenotyping Keratinocytes / metabolism* Mites / immunology Skin / metabolism, pathology Skin Tests |
| Chemical | |
Reg. No./Substance:
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0/Allergens; 0/Antigens, Dermatophagoides; 0/Glycoproteins; 0/HLA-DR Antigens; 9013-72-3/Hemocyanin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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