Document Detail


HLA-DR expression is induced on keratinocytes in delayed hypersensitivity but not in allergen induced late-phase reactions.
MedLine Citation:
PMID:  7788573     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In view of increasing evidence suggesting an active immunoregulatory role of the skin keratinocytes and the observation that the differentiation of allergen specific T lymphocytes is critical in the development of allergy, we evaluated epidermal expression of HLA-DR antigen in skin reactions induced with an atopen (house dust mite) and with an non-atopic antigen (Hemocyanin). Two groups of patients with house dust mite (Dermatophagoides pteronyssinus [Der p]) allergy were compared, one group was skin tested with Der p, the other group was immunized and subsequently skin tested with Helix pomatia Hemocyanin (HPH). Biopsy specimens taken at 48 h after the HPH (n = 11) and Der p (n = 11) tests were analysed immunohistologically. Reactions in both groups were comparable in size. Immunohistological analysis showed domination by CD4+ lymphocytes. Expression of HLA-DR antigen by epidermal keratinocytes was observed in six out of 11 of the HPH induced reactions, but in none of the Der p induced reactions. Eosinophils were spotted only throughout the Der p induced reactions, showing a good correlation with the number of CD4 positive lymphocytes. The lack of HLA-DR expression by keratinocytes during the allergen-induced reaction, compared with the Hemocyanin induced reaction can be the result of a difference in cytokine profile of the lymphocytes dominating the dermal infiltrate. On the other hand evidence exists that defective HLA-DR expression by keratinocytes enhances antigen induced lymphocyte activation, and may thus contribute to the development of allergen-specific T-lymphocytes.
Authors:
F R Weller; M C De Jong; M S Weller; K Heeres; J G De Monchy; H M Jansen
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology     Volume:  25     ISSN:  0954-7894     ISO Abbreviation:  Clin. Exp. Allergy     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-07-24     Completed Date:  1995-07-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8906443     Medline TA:  Clin Exp Allergy     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  252-9     Citation Subset:  IM    
Affiliation:
Department of Pulmonology, University of Amsterdam, the Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Allergens / adverse effects
Animals
Antigens, Dermatophagoides
CD4-Positive T-Lymphocytes / immunology
Dermatitis, Allergic Contact / etiology,  metabolism*,  pathology
Eosinophils / immunology
Glycoproteins / adverse effects*
HLA-DR Antigens / biosynthesis*
Helix (Snails) / immunology
Hemocyanin / adverse effects*
Humans
Hypersensitivity, Delayed / etiology,  metabolism*,  pathology
Immunoenzyme Techniques
Immunophenotyping
Keratinocytes / metabolism*
Mites / immunology
Skin / metabolism,  pathology
Skin Tests
Chemical
Reg. No./Substance:
0/Allergens; 0/Antigens, Dermatophagoides; 0/Glycoproteins; 0/HLA-DR Antigens; 9013-72-3/Hemocyanin

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