| HLA DR-DQ Genotyping by Capillary Electrophoresis for Risk Assessment for Celiac Disease. | |
| | |
MedLine Citation:
|
PMID: 22976110 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
The risk for celiac disease (CD) is clearly related to specific HLA DQA1 and DQB1 alleles, but HLA -typing is often considered too costly for frequent use.Here we present a method using sequence-specific primed PCR (PCR-SSP) for HLA-DR-DQ genotyping optimized for capillary electrophoresis on Applied Biosystems 3130xl Genetic Analyzer. Requiring a total of three PCR reactions and a single electrophoretic step, this method reduces the reagent expenses and technical time for directed HLA typing to distinguish risk alleles for CD, with a sufficient throughput for large-scale screening projects. |
| | |
Authors:
|
Ewa H Lavant; Joyce Carlson |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Methods in molecular biology (Clifton, N.J.) Volume: 919 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
|
Created Date: 2012-09-14 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 297-307 Citation Subset: IM |
Affiliation:
|
Department of Biomedical Laboratory Science, Malmö University, Malmö, Sweden, Ewa.Lavant@skane.se. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Analysis of microsatellite instability by microfluidic-based electrophoresis.
Next Document: Anxiety-related behavior of orphan chimpanzees (Pan troglodytes schweinfurthii) at Gombe National Pa...