Document Detail


HLA-DO. A useful marker to distinguish florid follicular hyperplasia from follicular lymphoma by flow cytometry.
MedLine Citation:
PMID:  12817432     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
HLA-DO expression is regulated during B-cell development and activation. To determine whether the level of HLA-DO expression is a helpful marker in the differential diagnosis between florid follicular hyperplasia and follicular lymphoma, we analyzed single-cell suspensions from 29 lymph node specimens by 4-color flow cytometry. Of the 29 specimens, 7 were from patients with florid follicular hyperplasia, in which the clonality of the germinal center B cells (GCBCs) was indeterminate by flow cytometric immunophenotyping. The remaining 22 cases were follicular lymphomas; neoplastic cells in 2 of these cases lacked expression of surface immunoglobulin light chains. The level of HLA-DO expression in the reactive CD10+ GCBCs in florid follicular hyperplasia was markedly down-regulated compared with the CD10- polytypic B cells of the same specimens. In contrast, the level of HLA-DO expression in the CD10+ neoplastic cells in all cases of follicular lymphomas was similar to that of the CD10- polytypic B cells, thus higher than the reactive CD10+ GCBCs. This difference was confirmed by immunohistochemical staining. Our results suggest that HLA-DO is a useful marker to differentiate florid follicular hyperplasia from follicular lymphoma by flow cytometry, particularly when clonality of the CD10+ B cells is in question.
Authors:
Xinjian Chen; Peter E Jensen; Shiyong Li
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of clinical pathology     Volume:  119     ISSN:  0002-9173     ISO Abbreviation:  Am. J. Clin. Pathol.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-06-23     Completed Date:  2003-07-02     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370470     Medline TA:  Am J Clin Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  842-51     Citation Subset:  AIM; IM    
Affiliation:
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA, USA.
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MeSH Terms
Descriptor/Qualifier:
B-Lymphocytes / pathology*
Diagnosis, Differential
Flow Cytometry
HLA-D Antigens / analysis*
Hyperplasia
Immunohistochemistry
Immunophenotyping
Lymph Nodes / pathology
Lymphoma, Follicular / chemistry,  diagnosis*,  immunology
Neprilysin / analysis
Tumor Markers, Biological / analysis*
Grant Support
ID/Acronym/Agency:
AI30554/AI/NIAID NIH HHS; AI33614/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/HLA-D Antigens; 0/HLA-DO antigens; 0/Tumor Markers, Biological; EC 3.4.24.11/Neprilysin

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