| HLA-DO. A useful marker to distinguish florid follicular hyperplasia from follicular lymphoma by flow cytometry. | |
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MedLine Citation:
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PMID: 12817432 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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HLA-DO expression is regulated during B-cell development and activation. To determine whether the level of HLA-DO expression is a helpful marker in the differential diagnosis between florid follicular hyperplasia and follicular lymphoma, we analyzed single-cell suspensions from 29 lymph node specimens by 4-color flow cytometry. Of the 29 specimens, 7 were from patients with florid follicular hyperplasia, in which the clonality of the germinal center B cells (GCBCs) was indeterminate by flow cytometric immunophenotyping. The remaining 22 cases were follicular lymphomas; neoplastic cells in 2 of these cases lacked expression of surface immunoglobulin light chains. The level of HLA-DO expression in the reactive CD10+ GCBCs in florid follicular hyperplasia was markedly down-regulated compared with the CD10- polytypic B cells of the same specimens. In contrast, the level of HLA-DO expression in the CD10+ neoplastic cells in all cases of follicular lymphomas was similar to that of the CD10- polytypic B cells, thus higher than the reactive CD10+ GCBCs. This difference was confirmed by immunohistochemical staining. Our results suggest that HLA-DO is a useful marker to differentiate florid follicular hyperplasia from follicular lymphoma by flow cytometry, particularly when clonality of the CD10+ B cells is in question. |
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Authors:
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Xinjian Chen; Peter E Jensen; Shiyong Li |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: American journal of clinical pathology Volume: 119 ISSN: 0002-9173 ISO Abbreviation: Am. J. Clin. Pathol. Publication Date: 2003 Jun |
Date Detail:
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Created Date: 2003-06-23 Completed Date: 2003-07-02 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370470 Medline TA: Am J Clin Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 842-51 Citation Subset: AIM; IM |
Affiliation:
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Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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B-Lymphocytes
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pathology* Diagnosis, Differential Flow Cytometry HLA-D Antigens / analysis* Hyperplasia Immunohistochemistry Immunophenotyping Lymph Nodes / pathology Lymphoma, Follicular / chemistry, diagnosis*, immunology Neprilysin / analysis Tumor Markers, Biological / analysis* |
| Grant Support | |
ID/Acronym/Agency:
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AI30554/AI/NIAID NIH HHS; AI33614/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/HLA-D Antigens; 0/HLA-DO antigens; 0/Tumor Markers, Biological; EC 3.4.24.11/Neprilysin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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