Document Detail


HLA-D region beta-chain DNA endonuclease fragments differ between HLA-DR identical healthy and insulin-dependent diabetic individuals.
MedLine Citation:
PMID:  6306468     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The human HLA-D histocompatibility region encodes class II antigens each of which consists of two polypeptide chains (alpha and beta) inserted in the plasma membrane. These molecules are implicated in the regulation of the immune response but several human diseases are also found to be associated with certain HLA-DR antigens. The occurrence of insulin-dependent (type I) diabetes (IDDM) is strongly associated with HLA-DR3 and/or 4 (ref. 5). The class II antigens, however, show a marked genetic polymorphism associated with the beta-chains which seem, from hybridization studies, to be encoded by several genes. We have therefore used the beta-chain cDNA probe, pDR-beta-1 (refs 8, 10) to test whether there are differences in hybridization pattern between DNA from healthy individuals and diabetic patients, after digestion with restriction endonucleases. Among the HLA-DR 4 and 3/4 individuals, the IDDM patients showed an increased frequency of a PstI 18 kilobase (kb) fragment. A BamHI 3.7 kb fragment, frequent among controls (30-40%), was rarely detected in the IDDM patients (0-2%). These differences may be related to susceptibility to develop the disease.
Authors:
D Owerbach; A Lernmark; P Platz; L P Ryder; L Rask; P A Peterson; J Ludvigsson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nature     Volume:  303     ISSN:  0028-0836     ISO Abbreviation:  Nature     Publication Date:  1983 Jun 
Date Detail:
Created Date:  1983-08-11     Completed Date:  1983-08-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0410462     Medline TA:  Nature     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  815-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
DNA Restriction Enzymes
Diabetes Mellitus / genetics*
Gene Frequency
Genes, MHC Class II*
Humans
Major Histocompatibility Complex
Nucleic Acid Hybridization
Chemical
Reg. No./Substance:
EC 3.1.21.-/DNA Restriction Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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