Document Detail


HLA-B*51 allele analysis by the PCR-SBT method and a strong association of HLA-B*5101 with Japanese patients with Behçet's disease.
MedLine Citation:
PMID:  11703826     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Behçet's disease (BD) is known to be associated with human leukocyte antigen (HLA) B51 in many different ethnic groups. An increased incidence of HLA-B51 in the patient group has also been reported in a Japanese population. Recently, the B51 antigen has been identified to comprise 21 alleles, B*5101-B*5121. Further, not only HLA-B*5101 but also HLA-B*5108 were found to be relatively increased in the patient groups among Italian and Saudi Arabian populations. Therefore, we performed HLA-B*51 allele genotyping by the polymerase chain reaction-sequencing based typing (PCR-SBT) method in order to investigate whether there is any correlation of one particular B51-associated allele with Japanese BD. Ninety-six Japanese patients with BD and 132 healthy Japanese volunteers were enrolled in this study. As a result, the phenotype frequency of the B51 antigen was confirmed to be remarkably increased in the patient group as compared to the ethnically matched control group (59.4% in patients vs. 13.6% in controls; Pc=0.0000000000098, R.R.=9.3). In the B*51 allele genotyping, 56 out of 57 B51-positive patients were defined as B*5101 and the remaining one was B*5102. In contrast, all of 18 B51-positive normal controls were B*5101. None of the Japanese patients and healthy controls carried the HLA-B*5108 allele. This study revealed that B*51 allelic distribution in Japanese was different from those in Italian and Saudi Arabian populations, and that the significantly high incidence of the HLA-B51 antigen in the Japanese BD patient group was mostly caused by the significant increase of the HLA-B*5101 allele.
Authors:
N Mizuki; M Ota; Y Katsuyama; K Yabuki; H Ando; T Shiina; E Nomura; K Onari; S Ohno; H Inoko
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Tissue antigens     Volume:  58     ISSN:  0001-2815     ISO Abbreviation:  Tissue Antigens     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-11-12     Completed Date:  2002-01-25     Revised Date:  2010-11-29    
Medline Journal Info:
Nlm Unique ID:  0331072     Medline TA:  Tissue Antigens     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  181-4     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology, Yokohama City University School of Medicine, Kanazawa-ku, Yokohama, Kanagawa, Japan.
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MeSH Terms
Descriptor/Qualifier:
Alleles*
Behcet Syndrome / genetics*
Gene Frequency
Genes, MHC Class I
Genetic Predisposition to Disease*
Genotype
HLA-B Antigens / genetics*
Histocompatibility Testing
Humans
Japan
Polymerase Chain Reaction / methods
Chemical
Reg. No./Substance:
0/HLA-B Antigens; 0/HLA-B51

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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