Document Detail


HIV-specific regulatory T cells are associated with higher CD4 cell counts in primary infection.
MedLine Citation:
PMID:  19005268     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Expansion of regulatory T (Treg) cells has been described in chronically HIV-infected individuals. We investigated whether HIV-suppressive Treg could be detected during primary HIV infection (PHI).
METHODS: Seventeen patients diagnosed early after PHI (median: 13 days; 1-55) were studied. Median CD4 cell count was 480 cells/microl (33-1306) and plasma HIV RNA levels ranged between 3.3 and 5.7 log10 copies/ml. Suppressive capacity of blood purified CD4CD25 was evaluated in a coculture assay. Fox-p3, IL-2 and IL-10 were quantified by reverse transcriptase (RT)-PCR and intracellular staining of ex vivo and activated CD4+CD25 T cells.
RESULTS: The frequency of CD4CD127CD25 T cells among CD4 T cells was lower in patients with PHI compared with chronic patients (n = 19). They exhibited a phenotype of memory T cells and expressed constitutively FoxP3. Similar to chronic patients, Treg from patients with PHI inhibited the proliferation of purified tuberculin (PPD) and HIV p24 activated CD4CD25 T cells. CD4CD25 T cells from patients with PHI responded specifically to p24 stimulation by expressing IL-10. In untreated patients with PHI, the frequency as well as HIV-specific activity of Treg decreased during a 24-month follow-up. A positive correlation between percentages of Treg and both CD4 cell counts and the magnitude of p24-specific suppressive activity at diagnosis of PHI was found.
CONCLUSION: Our data showed that HIV drives Treg, as PHI and these cells persist throughout the course of the infection. A correlation between the frequency of Treg and CD4 T-cell counts suggest that these cells may impact on the immune activation set point at PHI diagnosis.
Authors:
Hassen Kared; Jean-Daniel Lelièvre; Vladimira Donkova-Petrini; Albertine Aouba; Giovanna Melica; Michèle Balbo; Laurence Weiss; Yves Lévy
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Publication Detail:
Type:  Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  AIDS (London, England)     Volume:  22     ISSN:  1473-5571     ISO Abbreviation:  AIDS     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-11-13     Completed Date:  2009-02-20     Revised Date:  2013-06-04    
Medline Journal Info:
Nlm Unique ID:  8710219     Medline TA:  AIDS     Country:  England    
Other Details:
Languages:  eng     Pagination:  2451-60     Citation Subset:  IM; X    
Affiliation:
INSERM, Unite U841, France.
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MeSH Terms
Descriptor/Qualifier:
CD4 Lymphocyte Count / methods
Cell Proliferation
Female
Flow Cytometry
HIV Infections / diagnosis,  immunology*,  virology
HIV-1 / immunology*
Humans
Interleukin-10 / immunology
Interleukin-2 Receptor alpha Subunit / immunology
Male
Phenotype
Prospective Studies
RNA, Viral / immunology*,  metabolism
Suppressor Factors, Immunologic / immunology*,  metabolism
T-Lymphocytes, Regulatory / immunology*,  virology
Viral Load
Chemical
Reg. No./Substance:
0/Interleukin-2 Receptor alpha Subunit; 0/RNA, Viral; 0/Suppressor Factors, Immunologic; 130068-27-8/Interleukin-10
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