| HIV and HTLV infections in 1305 transfusion-dependent thalassemics in Italy. The COOLEYCARE Cooperative Group. | |
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MedLine Citation:
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PMID: 1352107 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To evaluate the prevalence of antibodies to HIV-1/2 and HTLV-I/II in 1305 transfusion-dependent beta-thalassemics treated in 36 centres in Italy. DESIGN: Patient serum samples were collected during 1990 and tested in Milan. METHODS: Sera were screened using an enzyme-linked immunosorbent assay (ELISA) containing viral lysate antigens from HIV-1 and HIV-2, and a particle agglutination assay for the detection of antibodies to HTLV-I and HTLV-II. Repeatedly reactive samples were examined by Western blot (WB) assays containing recombinant and viral lysate antigens. Differential diagnosis was finally made by ELISA based on synthetic peptides. RESULTS: Samples from 36 of the 1305 patients (2.76%) contained anti-HIV-1 antibodies. In four patients seroconversion occurred after the implementation of anti-HIV-1 screening in blood donors in Italy (1985). Of the 36 HIV-1-antibody-positive samples, four were HIV-2 [corrected] WB indeterminate. These four samples were negative in assays based on specific synthetic peptides, suggesting cross-reactivity. Anti-HTLV-I antibodies were found in two patients from Sicily and one from Apulia, both southern Italian regions. Anti-HTLV-II antibodies were detected in another patient from Sicily. CONCLUSIONS: Antibodies to HIV-1, HIV-2, HTLV-I and HTLV-II were detected in 2.76, 0, 0.23 and 0.08% of patients, respectively. The residual risk of HIV-1 infection through blood transfusion after the implementation of anti-HIV-1 screening in blood donors in Italy was approximately 1:50,000 blood units; this is based on an approximate number of 200,000 blood units administered to our group of patients during 1986-1990 and the occurrence of four new anti-HIV-1 seroconversions. Seroconversions to HTLV-I/II suggest that these viruses are present in Italian blood donors. |
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Authors:
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F Mozzi; P Rebulla; F Lillo; O E Varnier; C Biadati; L Calcagno; S Melotti; G Sirchia |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: AIDS (London, England) Volume: 6 ISSN: 0269-9370 ISO Abbreviation: AIDS Publication Date: 1992 May |
Date Detail:
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Created Date: 1992-08-06 Completed Date: 1992-08-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8710219 Medline TA: AIDS Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 505-8 Citation Subset: IM; X |
Affiliation:
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Centro Trasfusionale e di Immunologia dei Trapianti, Ospedale Maggiore Policlinico, Milano, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Blood Transfusion / adverse effects* Child Child, Preschool Deltaretrovirus Infections / complications, epidemiology* HIV Infections / complications, epidemiology* Humans Infant Italy / epidemiology Thalassemia / complications*, epidemiology, therapy |
| Comments/Corrections | |
Erratum In:
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AIDS 1992 Jun;6(6):following 606 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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