Document Detail


HIV-2 genomic RNA accumulates in stress granules in the absence of active translation.
MedLine Citation:
PMID:  25352557     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
During the post-transcriptional events of the HIV-2 replication cycle, the full-length unspliced genomic RNA (gRNA) is first used as an mRNA to synthesize Gag and Gag-Pol proteins and then packaged into progeny virions. However, the mechanisms responsible for the coordinate usage of the gRNA during these two mutually exclusive events are poorly understood. Here, we present evidence showing that HIV-2 expression induces stress granule assembly in cultured cells. This contrasts with HIV-1, which interferes with stress granules assembly even upon induced cellular stress. Moreover, we observed that the RNA-binding protein and stress granules assembly factor TIAR associates with the gRNA to form a TIAR-HIV-2 ribonucleoprotein (TH2RNP) complex localizing diffuse in the cytoplasm or aggregated in stress granules. Although the assembly of TH2RNP in stress granules did not require the binding of the Gag protein to the gRNA, we observed that increased levels of Gag promoted both translational arrest and stress granule assembly. Moreover, HIV-2 Gag also localizes to stress granules in the absence of a 'packageable' gRNA. Our results indicate that the HIV-2 gRNA is compartmentalized in stress granules in the absence of active translation prior to being selected for packaging by the Gag polyprotein.
Authors:
Ricardo Soto-Rifo; Fernando Valiente-Echeverria; Paulina S Rubilar; Francisco Garcia-de-Gracia; Emiliano P Ricci; Taran Limousin; Didier Décimo; Andrew J Mouland; Théophile Ohlmann
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-10-28
Journal Detail:
Title:  Nucleic acids research     Volume:  -     ISSN:  1362-4962     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  2014 Oct 
Date Detail:
Created Date:  2014-10-29     Completed Date:  -     Revised Date:  2014-10-30    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.
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