| HIV-1 replication and gene expression occur at higher levels in neonatal blood naive and memory T-lymphocytes compared with adult blood cells. | |
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MedLine Citation:
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PMID: 21353282 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Our previous study has shown that HIV-1 replicated at higher levels in neonatal (cord) blood monocytes/macrophages and T-lymphocytes compared with adult blood cells. However, it is not known whether this differential HIV-1 replication also occurs in naive and/or memory T-lymphocytes. We, therefore, compared HIV-1 replication in CD3(+) and CD4(+) naive (CD45RA(+)) and memory (CD45RO(+)) T-lymphocytes isolated from five cord and adult blood donors. We found that HIV-1 replicated at higher levels in both CD3(+) and CD4(+) CD45RA(+) and CD45RO(+) T-lymphocytes isolated from cord blood compared with adult blood. In addition, there was no difference in the cell surface expression of CD4, CXCR4 and CCR5 on cord blood CD45RA(+) and CD45RO(+) T-lymphocytes compared with adult blood cells. Furthermore, we found that there was an increase in HIV-1 gene expression in cord blood CD45RA(+) and CD45RO(+) T-lymphocytes compared with adult blood cells by using a single-cycle replication competent HIV-1-NL4-3-Env(-)R(+) luciferase amphotropic virus, which measures HIV-1 transcriptional activity independent of CD4 and CXCR4 or CCR5 expression. In summary, HIV-1 replicated at higher levels in cord blood CD45RA(+) and CD45RO(+) T-lymphocytes compared with adult blood cells and this differential replication is influenced at the level of HIV-1 gene expression. |
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Authors:
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Nafees Ahmad; Roshni Mehta; David T Harris |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-2-24 |
Journal Detail:
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Title: Virology Volume: - ISSN: 1096-0341 ISO Abbreviation: - Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-2-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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