| HIV-1 proviral DNA excision using an evolved recombinase. | |
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MedLine Citation:
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PMID: 17600219 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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HIV-1 integrates into the host chromosome and persists as a provirus flanked by long terminal repeats (LTRs). To date, treatment regimens primarily target the virus enzymes or virus-cell fusion, but not the integrated provirus. We report here the substrate-linked protein evolution of a tailored recombinase that recognizes an asymmetric sequence within an HIV-1 LTR. This evolved recombinase efficiently excised integrated HIV proviral DNA from the genome of infected cells. Although a long way from use in the clinic, we speculate that this type of technology might be adapted in future antiretroviral therapies, among other possible uses. |
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Authors:
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Indrani Sarkar; Ilona Hauber; Joachim Hauber; Frank Buchholz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Science (New York, N.Y.) Volume: 316 ISSN: 1095-9203 ISO Abbreviation: Science Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-06-29 Completed Date: 2007-07-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0404511 Medline TA: Science Country: United States |
Other Details:
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Languages: eng Pagination: 1912-5 Citation Subset: IM |
Affiliation:
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Max-Planck-Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D-01307 Dresden, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Base Sequence DNA Shuffling DNA, Viral / metabolism* Directed Molecular Evolution* Escherichia coli / genetics Gene Library Genome, Human HIV Long Terminal Repeat* HIV-1 / metabolism* Hela Cells Humans Integrases / genetics*, metabolism* Molecular Sequence Data Mutation Proviruses / metabolism Recombination, Genetic Virus Integration* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral; EC 2.7.7.-/Cre recombinase; EC 2.7.7.-/Integrases |
| Comments/Corrections | |
Comment In:
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Science. 2007 Jun 29;316(5833):1855-7
[PMID:
17600205
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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