Document Detail


HIV-1 integration site preferences in pluripotent cells.
MedLine Citation:
PMID:  21196325     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
HIV-1-based vectors are widely used in gene therapy. In somatic cells, these vectors mainly integrate within genes. However, no distinct integration site preferences have been observed with regard to large chromosomal regions. The recent emergence of induced pluripotent stem (iPS) cells, similar to embryonic stem (ES) cells, has raised questions about where integration occurs in these cells. In this work we investigated the integration site preferences of HIV-1-based vectors in a pluripotent, ES-like cell line. We show that approximately 30% of the integrations occur in the vicinity of telomeres. We have analyzed integration sites in various somatic cells, as reported by us and other groups, and observed that this integration pattern is unique to the analyzed pluripotent cell line. We conclude that pluripotent cells may contain distinct cellular cofactors that participate in integration targeting and that are not present in somatic cells.
Authors:
Janet L Markman; Robert M Silvers; Abibatou M N Ndoye; Kyla R Geary; David Alvarado; Johanna A Smith; Rene Daniel
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-01-01
Journal Detail:
Title:  Frontiers in bioscience (Elite edition)     Volume:  3     ISSN:  1945-0508     ISO Abbreviation:  Front Biosci (Elite Ed)     Publication Date:  2011  
Date Detail:
Created Date:  2011-01-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101485240     Medline TA:  Front Biosci (Elite Ed)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  453-62     Citation Subset:  IM    
Affiliation:
Division of Infectious Diseases-Center for Human Virology, Thomas Jefferson University, Philadelphia, PA, USA.
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MeSH Terms
Descriptor/Qualifier:
Grant Support
ID/Acronym/Agency:
CA125272/CA/NCI NIH HHS; CA135214/CA/NCI NIH HHS

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