Document Detail


HIV-1 gp120 induces the activation of both c-fos and c-jun immediate-early genes in HEL megakaryocytic cells.
MedLine Citation:
PMID:  10027715     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have previously demonstrated that the addition in culture of recombinant HIV-1 IIIB envelope gp120 affects the survival/growth of pluripotent haemopoietic progenitors, and, in particular, of those committed towards the megakaryocytic lineage. To characterize some of the molecular mechanisms involved in this phenomenon, we investigated the expression of members of the activating protein-1 (AP-1) complex in the HEL megakaryoblastic cell line. Following the treatment of HEL cells with recombinant IIIB envelope gp120, we noticed: (i) increased levels of endogenous c-fos and c-jun mRNA and proteins, (ii) activation of both c-fos and c-jun promoters, and (iii) a very rapid stimulation of a MAPK/ERK pathway.
Authors:
D Gibellini; M C Re; A Bassini; L Guidotti; L Catani; M La Placa; G Zauli
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  British journal of haematology     Volume:  104     ISSN:  0007-1048     ISO Abbreviation:  Br. J. Haematol.     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-03-22     Completed Date:  1999-03-22     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0372544     Medline TA:  Br J Haematol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  81-6     Citation Subset:  IM; X    
Affiliation:
Department of Clinical and Experimental Medicine, University of Bologna, Italy.
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Gene Expression Regulation
Genes, fos / genetics*
Genes, jun / genetics*
HIV Envelope Protein gp120 / pharmacology*
HIV-1*
Hematopoietic Stem Cells / metabolism*
Humans
Megakaryocytes / metabolism*
Proto-Oncogene Proteins c-fos / metabolism
Proto-Oncogene Proteins c-jun / metabolism
RNA, Messenger / metabolism
Transcription Factor AP-1
Transcription, Genetic
Chemical
Reg. No./Substance:
0/HIV Envelope Protein gp120; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 0/RNA, Messenger; 0/Transcription Factor AP-1

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