Document Detail

HIV-1 epitopes exposed by hybrid hepatitis B core particles affect proliferation of peripheral blood mononuclear cells from HIV-1 positive donors.
MedLine Citation:
PMID:  8877417     Owner:  NLM     Status:  MEDLINE    
Hepatitis B virus core protein (HBc) in particulate form is a potent immunogen for the design of vaccines with a broad T-cell reactivity. Hybrid HBc proteins with N-terminal insertions of human immunodeficiency virus (HIV-1) B- and T-cell epitopes from gp41 and p34 pol, respectively, were constructed HBc hybrids formed particles with HIV-1 epitopes exposed on the surface. The proliferative response of peripheral blood mononuclear cells (PBMC) from HIV-1 infected donors to the hybrids was studied in vitro and compared to that of synthetic peptides representing the same HIV-1 sequences. The epitope from p34 pol induced PBMC proliferation both when inserted into HBc and as a peptide. The epitope from gp41, when inserted into HBc, and to a lesser extent the relevant peptide caused a decreased reactivity. The inhibitory effect of the HBc hybrid carrying the gp41-epitope was pronounced even in the HIV-1 infected donors with proliferative responses to HBc. The similarity between the mode of action of the peptides and hybrid HBcs implies either correct processing of the latter or T-cell recognition of HIV-1 epitopes in the intact hybrid HBc particles.
M G Isaguliants; S Nordlund; M Sällberg; V D Smirnov; U Rudén; B Wahren
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Immunology letters     Volume:  52     ISSN:  0165-2478     ISO Abbreviation:  Immunol. Lett.     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1997-02-06     Completed Date:  1997-02-06     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  7910006     Medline TA:  Immunol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  37-44     Citation Subset:  IM; X    
Swedish Institute for Infectious Disease Control, Stockholm, Sweden.
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MeSH Terms
Amino Acid Sequence
Gene Products, pol / genetics,  immunology
HIV Envelope Protein gp41 / genetics,  immunology
HIV Seropositivity / immunology*
HIV-1 / immunology*
Hepatitis B Core Antigens / genetics,  immunology*
Leukocytes, Mononuclear / immunology*
Molecular Sequence Data
Recombinant Fusion Proteins / immunology
Reg. No./Substance:
0/Epitopes; 0/Gene Products, pol; 0/HIV Envelope Protein gp41; 0/Hepatitis B Core Antigens; 0/Recombinant Fusion Proteins
Erratum In:
Immunol Lett 1997 Jan;55(1):61

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