Document Detail


HIV-1 envelope protein, gp120, has no effects on inositol phosphate production and metabolism in the Jurkat T-cell line either in the presence or absence of receptor stimulation.
MedLine Citation:
PMID:  9287120     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have used HPLC techniques to investigate the effects of gp120 upon inositol phosphate turnover in Jurkat E6-1 CD4+ T-cells, to pursue previous reports that this viral coat protein: (a) inhibits receptor-activated inositol phosphate release; (b) stimulates basal inositol phosphate release; (c) inhibits inositol polyphosphate 5-phosphatase. Treatment of cells with up to 10 microg/ml gp120 from between 10 min and 24 h was without effect upon inositol phosphate turnover in both basal cells, and in C305 and OKT3 stimulated cells. This is the first report that biologically competent gp120 does not affect any aspect of inositol phosphate turnover in either basal or receptor-activated lymphocytes.
Authors:
M T Sumner; S B Shears
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  413     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1997-10-30     Completed Date:  1997-10-30     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  75-80     Citation Subset:  IM; X    
Affiliation:
Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA. sumner1@niehs.nih.gov
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MeSH Terms
Descriptor/Qualifier:
Chromatography, High Pressure Liquid
HIV Envelope Protein gp120 / isolation & purification,  pharmacology*
Humans
Inositol Phosphates / biosynthesis*
Jurkat Cells / metabolism
Phosphoric Monoester Hydrolases / metabolism
Recombinant Proteins / pharmacology
Signal Transduction / drug effects
Time Factors
Type C Phospholipases / metabolism
Chemical
Reg. No./Substance:
0/HIV Envelope Protein gp120; 0/Inositol Phosphates; 0/Recombinant Proteins; EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC 3.1.3.56/inositol-1,4,5-trisphosphate 5-phosphatase; EC 3.1.4.-/Type C Phospholipases

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