| HIV-1 Vpu's lipid raft association is dispensable for counteraction of the particle release restriction imposed by CD317/Tetherin. | |
| | |
MedLine Citation:
|
PMID: 22222210 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
HIV-1 Vpu antagonizes the block to particle release mediated by CD317 (BST-2/HM1.24/Tetherin) via incompletely understood mechanisms. Vpu and CD317 partially reside in cholesterol-rich lipid rafts where HIV-1 budding preferentially occurs. Here we find that lipid raft association of ectopically expressed or endogenous CD317 was unaltered upon co-expression with Vpu or following HIV-1 infection. Similarly, Vpu's lipid raft association remained unchanged upon expression of CD317. We identify amino acids V25 and Y29 of Vpu as crucial for microdomain partitioning and single substitution of these amino acids resulted in Vpu variants with markedly reduced or undetectable lipid raft association. These mutations did not affect Vpu's subcellular distribution and binding capacity to CD317, nor its ability to downmodulate cell surface CD317 and promote HIV-1 release from CD317-positive cells. We conclude that (i) lipid raft incorporation is dispensable for Vpu-mediated CD317 antagonism and (ii) Vpu does not antagonize CD317 by extraction from lipid rafts. |
| | |
Authors:
|
Joëlle V Fritz; Nadine Tibroni; Oliver T Keppler; Oliver T Fackler |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-1-3 |
Journal Detail:
|
Title: Virology Volume: - ISSN: 1096-0341 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2012-1-6 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0110674 Medline TA: Virology Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 Elsevier Inc. All rights reserved. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Glycosyl-phosphatidylinositol (GPI)-anchored membrane association of the porcine reproductive and re...
Next Document: Cathepsins B and L activate Ebola but not Marburg virus glycoproteins for efficient entry into cell ...