| HIV-1 Tat regulates cyclin B1 by promoting both expression and degradation. | |
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MedLine Citation:
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PMID: 19825974 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cyclin B1, an important cell cycle regulator, was up-regulated in lymphocytes of human immunodeficiency virus (HIV)-infected patients. However, the mechanism of cyclin B1 up-regulation and the effects of the up-regulation on the host cells remain unclear. Here, we show that HIV-encoded Tat protein regulates cyclin B1 levels in two different ways: first, Tat stimulates the transcription of cyclin B1, which increases cyclin B1 levels and promotes the cells apoptosis; and second, Tat stimulates polyubiquitination-mediated degradation of cyclin B1 through binding to the N-terminal of cyclin B1 (aa 61-129) that is just downstream of the D box, which prevents excessive levels of cyclin B1 in the cells. These results suggest that Tat-regulating cyclin B1 affects the status of HIV: Tat stimulates cyclin B1 expression to slow down the host cell cycle progress and to promote the host cell apoptosis, which might facilitate HIV release; Tat stimulates cyclin B1 degradation to prevent overaccumulation of cyclin B1, which might facilitate HIV replication. Taken together, our results reveal for the first time how HIV-Tat regulates cyclin B1 and keeps its balance in the cells. |
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Authors:
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Shi-Meng Zhang; Yi Sun; Rong Fan; Qin-Zhi Xu; Xiao-Dan Liu; Xiangming Zhang; Ya Wang; Ping-Kun Zhou |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-10-13 |
Journal Detail:
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Title: FASEB journal : official publication of the Federation of American Societies for Experimental Biology Volume: 24 ISSN: 1530-6860 ISO Abbreviation: FASEB J. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-01-29 Completed Date: 2010-02-25 Revised Date: 2013-05-31 |
Medline Journal Info:
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Nlm Unique ID: 8804484 Medline TA: FASEB J Country: United States |
Other Details:
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Languages: eng Pagination: 495-503 Citation Subset: IM |
Affiliation:
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Department of Radiation Toxicology and Oncology, Beijing Institute of Radiation Medicine, Beijing, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Cell Line, Tumor Cyclin B1 / biosynthesis, metabolism* Gene Expression HIV Infections / genetics, physiopathology Humans Transcription, Genetic / drug effects Up-Regulation / drug effects tat Gene Products, Human Immunodeficiency Virus / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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G-M080771//PHS HHS |
| Chemical | |
Reg. No./Substance:
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0/Cyclin B1; 0/tat Gene Products, Human Immunodeficiency Virus |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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