Document Detail


HIV-1 infection impairs HSV-specific CD4(+) and CD8(+) T-cell response by reducing Th1 cytokines and CCR5 ligand secretion.
MedLine Citation:
PMID:  21646911     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The concept of HIV-1/HSV-negative immunosynergy has recently come to light, which leads us to explore the impact of HIV-1 infection on HSV-specific T-cell immunity.
METHODS: : A combination of interferon (IFN)-γ ELISpot and Luminex-based multicytokine profiling assays was used to compare, in a cross-sectional study, the HSV-specific CD4 and CD8 T-cell responses between 20 HIV-1/HSV-coinfected and 12 HIV-1-uninfected/HSV-infected individuals after in vitro restimulation with HSV glycoprotein D (gD) peptide epitopes.
RESULTS: In response to CD4 and CD8 gD peptide epitopes, mean value (±standard errors of the mean) of the different IFN-γ-secreting T cells (ISC) means was significantly reduced in HIV-1/HSV-coinfected individuals (70 ISC ± 10 and 60 ISC ± 8/10 cells) compared with HIV-1-uninfected/HSV-infected individuals (280 ISC ± 25 and 234 ISC ± 23/10 cells, both P < 0.001). After stimulation with the immunodominant CD4 gD and CD8 gD peptide epitopes, the Th1 cytokine and CCR5 ligand secretions were decreased in the HIV-1-infected group although Th17 cytokines increased. The mean concentration of interleukin (IL)-2, IFN-γ, the IFN-γ-induced protein 10 kDa, and the monokine induced by IFN-γ was correlated to the mean concentration of macrophage inflammatory proteins (MIP-1α, MIP-1β), RANTES and Eotaxin (ρ = 0.56, P = 0.02 and ρ = 0.52, P = 0.03).
CONCLUSIONS: HIV-1 infection impairs both the number and function of HSV-specific T cells. The downregulation of Th1 cytokines and CCR5 ligands in HIV-1/HSV-coinfected individuals may further facilitate both HSV reactivations and HIV-1 replication.
Authors:
Pierre-Alain Rubbo; Edouard Tuaillon; Nicolas Nagot; Aziz Alami Chentoufi; Karine Bolloré; Jacques Reynes; Jean-Pierre Vendrell; Lbachir BenMohamed; Philippe Van De Perre
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of acquired immune deficiency syndromes (1999)     Volume:  58     ISSN:  1944-7884     ISO Abbreviation:  J. Acquir. Immune Defic. Syndr.     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-08-22     Completed Date:  2011-11-02     Revised Date:  2012-04-24    
Medline Journal Info:
Nlm Unique ID:  100892005     Medline TA:  J Acquir Immune Defic Syndr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  9-17     Citation Subset:  IM; X    
Affiliation:
Université Montpellier 1, UMR 1058, 34967 Montpellier, France. pierrealainrubbo@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Adult
CD4-Positive T-Lymphocytes / metabolism,  physiology*
CD8-Positive T-Lymphocytes / metabolism,  physiology*
Cytokines / metabolism*
Down-Regulation
Epitopes / immunology
Female
HIV Infections / complications,  immunology*,  virology
HIV-1*
Herpes Simplex / complications,  immunology
Humans
Ligands
Male
Middle Aged
Receptors, CCR5 / metabolism
Simplexvirus / immunology*
Th1 Cells / immunology
Viral Proteins / immunology
Grant Support
ID/Acronym/Agency:
EY014900/EY/NEI NIH HHS; EY019896/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Cytokines; 0/Epitopes; 0/Ligands; 0/Receptors, CCR5; 0/Viral Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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