Document Detail

HIF and VEGF relationships in response to hypoxia and sciatic nerve stimulation in rat gastrocnemius.
MedLine Citation:
PMID:  15522704     Owner:  NLM     Status:  MEDLINE    
To determine if hypoxia-inducible factor-1 (HIF-1) may regulate skeletal muscle vascular endothelial growth factor (VEGF) expression in response to exercise or hypoxia, rats underwent 1h sciatic nerve electrical stimulation (ES), hypoxic exposure (H) or combined stimuli. HIF-1alpha protein levels increased six-fold with maximal (8V) ES with or without H. Similar HIF-1alpha increases occurred with sub-maximal (6V and 4V) ES plus H, but not in sub-maximal ES or H alone. VEGF mRNA and protein levels increased three-fold in sub-maximal ES or H alone, six-fold in sub-maximal ES plus H, 6.3-fold with maximal ES, and 6.5-fold after maximal ES plus H. These data suggest: (1) intracellular hypoxia during normoxic exercise may exceed that during 8% oxygen breathing at rest and is more effective in stimulating HIF-1alpha; (2) HIF-1 may be an important regulator of exercise-induced VEGF transcription; and (3) breathing 8% O(2) does not alter HIF-1alpha expression in skeletal muscle, implying that exercise-generated signals contribute to the regulation of HIF-1alpha and/or VEGF.
Kechun Tang; Ellen C Breen; Harrieth Wagner; Tom D Brutsaert; Max Gassmann; Peter D Wagner
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Respiratory physiology & neurobiology     Volume:  144     ISSN:  1569-9048     ISO Abbreviation:  Respir Physiol Neurobiol     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2004-11-03     Completed Date:  2005-02-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  101140022     Medline TA:  Respir Physiol Neurobiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  71-80     Citation Subset:  IM    
Division of Physiology (0623A), Department of Medicine, University of California at San Diego, La Jolla, CA 92093, USA.
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MeSH Terms
Anoxia / metabolism*
DNA-Binding Proteins / genetics,  metabolism*
Electric Stimulation
Gene Expression Regulation
Hypoxia-Inducible Factor 1
Hypoxia-Inducible Factor 1, alpha Subunit
Muscle Contraction / physiology
Muscle, Skeletal / innervation,  metabolism*
Nuclear Proteins / genetics,  metabolism*
RNA, Messenger / analysis
Rats, Wistar
Reference Values
Sciatic Nerve / physiology*
Transcription Factors / genetics,  metabolism*
Vascular Endothelial Growth Factor A / genetics,  metabolism*
Grant Support
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Hif1a protein, rat; 0/Hypoxia-Inducible Factor 1; 0/Hypoxia-Inducible Factor 1, alpha Subunit; 0/Nuclear Proteins; 0/RNA, Messenger; 0/Transcription Factors; 0/Vascular Endothelial Growth Factor A

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