| HIF-1α Promotes A Hypoxia-Independent Cell Migration. | |
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MedLine Citation:
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PMID: 20882121 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Hypoxia-inducible factor-1α (HIF-1α) is known as a transactivator for VEGF gene promoter. It can be induced by hypoxia. However, no study has been done so far to dissect HIF-1α-mediated effects from hypoxia or VEGF-mediated effects. By using a HIF-1α knockout (HIF-1α KO) cell system in mouse embryonic fibroblast (MEF) cells, this study analyzes cell migration and HIF-1α, hypoxia and VEGF activation. A hypoxia-mediated HIF-1α induction and VEGF transactivation were observed: both HIF-1α WT lines had significantly increased VEGF transactivation, as an indicator for HIF-1α induction, in hypoxia compared to normoxia; in contrast, HIF-1α KO line had no increased VEGF transactivation under hypoxia. HIF-1α promotes cell migration: HIF-1α-KO cells had a significantly reduced migration compared to that of the HIF-1α WT cells under both normoxia and hypoxia. The significantly reduced cell migration in HIF-1α KO cells can be partially rescued by the restoration of WT HIF-1α expression mediated by adenoviral-mediated gene transfer. Interestingly, hypoxia has no effect on cell migration: the cells had a similar cell migration rate under hypoxic and normoxic conditions for both HIF-1α WT and HIF-1α KO lines, respectively. Collectively, these data suggest that HIF-1α plays a role in MEF cell migration that is independent from hypoxia-mediated effects. |
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Authors:
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Liyuan Li; Chikezie O Madu; Andrew Lu; Yi Lu |
Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: The open biology journal Volume: 3 ISSN: 1874-1967 ISO Abbreviation: - Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-9-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101537085 Medline TA: Open Biol J Country: - |
Other Details:
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Languages: ENG Pagination: 8-14 Citation Subset: - |
Affiliation:
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Department of Pathology and Laboratory Medicine, Memphis, TN. |
Export Citation:
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Descriptor/Qualifier:
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| Grant Support | |
ID/Acronym/Agency:
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R01 CA107162-04//NCI NIH HHS; R01 CA107162-04S1//NCI NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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