Document Detail


HDAC6 is required for invadopodia activity and invasion by breast tumor cells.
MedLine Citation:
PMID:  20970878     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Invasion across tissue boundaries by metastatic tumor cells depends on the proteolytic degradation of the extracellular matrix, initiated by the formation of invadopodia, actin-driven membrane protrusions with matrix-degradative activity. Yet, mechanisms underlying invadopodia formation remain largely unknown. In this report, we examined the role of the histone deacetylase HDAC6 in invadopodia formation and invasion by breast cancer cells. Using small interfering RNA silencing of protein expression in highly invasive MDA-MB-231 breast adenocarcinoma cells, we show that HDAC6 is required for two-dimensional matrix proteolysis. In addition, we demonstrate that HDAC6 acts as a tubulin and cortactin deacetylase. We also report that the inhibition of HDAC6 by siRNA or treatment with HDAC inhibitor TSA results in a decreased invasion capacity of a three-dimensional type I collagen matrix by MDA-MB-231 cells. These data identify HDAC6 as a critical component of the invasive apparatus of tumor cells, in both two- and three-dimensional matrices.
Authors:
Mercedes Rey; Marie Irondelle; François Waharte; Floria Lizarraga; Philippe Chavrier
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Publication Detail:
Type:  Journal Article     Date:  2010-10-23
Journal Detail:
Title:  European journal of cell biology     Volume:  90     ISSN:  1618-1298     ISO Abbreviation:  Eur. J. Cell Biol.     Publication Date:    2011 Feb-Mar
Date Detail:
Created Date:  2011-01-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7906240     Medline TA:  Eur J Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  128-35     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier GmbH. All rights reserved.
Affiliation:
Institut Curie, Research Center, 26 rue d'Ulm, 75248 Paris Cedex 05, France; Membrane and Cytoskeleton Dynamics, CNRS, UMR144, 26 rue d'Ulm, 75248 Paris Cedex 05, France.
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