| HDAC3 overexpression and colon cancer cell proliferation and differentiation. | |
| | |
MedLine Citation:
|
PMID: 17849419 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
An immunohistochemical analysis of human colorectal adenocarcinomas showed that cancer cells express widely varying levels of HDAC3. The SW480 colon cancer cell line was found to express high levels of HDAC3 compared to other colon cancer cell lines. p21 was poorly induced in SW480 cells relative to the lower HDAC3-expressing HT-29 cells. RNAi-induced reduction of HDAC3 in SW480 cells increased their constitutive, butyrate-, TSA-, and TNF-alpha-induced expression of p21, but did not cause all the gene expression changes induced upon general histone deacetylase (HDAC) inhibition. SW480 cells with lower HDAC3 expression appeared to be poised for gene expression responses with increased histone H4-K12 acetylation, but not K5, K8, or K16 acetylation. Even though p21 was readily activated in HT29 cells, HDAC3 siRNA nonetheless stimulated p21 expression in these cells to a greater degree than HDAC1 and HDAC2 siRNA. SW480 cells with lower HDAC3 levels displayed an enhanced cell cycle arrest and growth inhibition by butyrate, but without changes in apoptosis or sensitivity to chemotherapeutic agents. As reported for other colon cancer cell lines, butyrate induced the rapid downregulation of the secretory cell differentiation markers mucin 2 and intestinal trefoil factor in SW480 cells. Interestingly, selective HDAC3 inhibition was sufficient to downregulate these genes. Our data support a central role for HDAC3 in regulating the cell proliferation and differentiation of colon cancer cells and suggest a potential mechanism by which colon cancers may become resistant to luminal butyrate. |
| | |
Authors:
|
Colleen C Spurling; Cassandra A Godman; Emily J Noonan; Theodore P Rasmussen; Daniel W Rosenberg; Charles Giardina |
Related Documents
:
|
15194229 - Hydrolysis of nuclear phospholipids in relation with proliferative state in uterine str... 12406559 - Apoptosis sensitivity is not correlated with sensitivity to proliferation inhibition by... 12748129 - Epigenetic characteristics and development of embryos cloned from donor cells treated b... 16106909 - Dynamic properties of germ line-specific lamin b3: the role of the shortened rod domain. 11181179 - Assembly into chromatin and subtype-specific transcriptional effects of exogenous linke... 2658809 - Nuclear uptake of monoclonal antibody to a surface glycoprotein and its effect on trans... 159019 - The pasteur effect and catabolite repression in an oxidative yeast, kluyveromyces lactis. 18331509 - Low-frequency electromagnetic field effects on functional groups in human skin keratino... 17466949 - Hedgehog is involved in prostate basal cell hyperplasia formation and its progressing t... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Molecular carcinogenesis Volume: 47 ISSN: 1098-2744 ISO Abbreviation: Mol. Carcinog. Publication Date: 2008 Feb |
Date Detail:
|
Created Date: 2008-01-23 Completed Date: 2008-03-31 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 8811105 Medline TA: Mol Carcinog Country: United States |
Other Details:
|
Languages: eng Pagination: 137-47 Citation Subset: IM |
Copyright Information:
|
(c) 2007 Wiley-Liss, Inc. |
Affiliation:
|
Department of Molecular & Cell Biology, University of Connecticut, Storrs, Connecticut, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetylation Adenocarcinoma / enzymology, pathology* Apoptosis Base Sequence Cell Differentiation* Cell Line, Tumor Cell Proliferation* Colorectal Neoplasms / enzymology, pathology* DNA Primers Histone Deacetylases / metabolism* Histones / metabolism Humans Immunohistochemistry Reverse Transcriptase Polymerase Chain Reaction |
| Grant Support | |
ID/Acronym/Agency:
|
CA-81428/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/DNA Primers; 0/Histones; EC 3.5.1.98/Histone Deacetylases; EC 3.5.1.98/histone deacetylase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The generalizability of the Buss-Perry Aggression Questionnaire.
Next Document: Reduced expression of the Rassf1a gene and its aberrant DNA methylation in pancreatic duct adenocarc...