Document Detail

HCV seroreactivity and detection of HCV RNA in cirrhotics.
MedLine Citation:
PMID:  10626131     Owner:  NLM     Status:  MEDLINE    
This study was carried out to determine the presence of HCV infection in cirrhotic patients and to compare their serologic profiles with detection of HCV RNA by RT-PCR. Liver function profiles were assessed and correlated with infection. Coinfection of HCV with HBV was studied in the Indian context Sera from 62 patients of biopsy confirmed cirrhosis and an equal number of asymptomatic controls were tested for HCV by two ELISA (third generation) kits and nested reverse transcription PCR using primers from the 5'NCR. Other tests included HBV serology (ELISA for HBsAg and anti-HBc) and liver function tests. Twenty-four (38.7%) cases were HCV infected, of which 17 (70.8%) had past exposure to HBV or were coinfected with HBV (either being chronically infected or carriers of HBV). There was no significant difference in the clinical and liver function profiles of HCV infected and uninfected cirrhotics. Similarly, no difference was observed in cases coinfected with both HCV and HBV compared with those infected with HCV alone. Although the difference between positivity of HCV RNA and of anti-HCV was not significant, HCV RNA and anti HCV were present together in only 7/62 (11.3%) cases. Thus testing for both antibody and HCV RNA would be more appropriate than either test alone. HBV infection was seen in about one-fourth of HCV infected cirrhotics. About one-fourth (25.8%) patients had non-B, non-C cirrhosis in whom testing for HCV variants and other recently characterized hepatitis viruses could be performed.
N Berry; A Chakravarti; U Das; P Kar; B C Das; M D Mathur
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Diagnostic microbiology and infectious disease     Volume:  35     ISSN:  0732-8893     ISO Abbreviation:  Diagn. Microbiol. Infect. Dis.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  2000-02-23     Completed Date:  2000-02-23     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8305899     Medline TA:  Diagn Microbiol Infect Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  209-13     Citation Subset:  IM    
Department of Microbiology, Maulana Azad Medical College, New Delhi, India.
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MeSH Terms
DNA Primers / chemistry
Enzyme-Linked Immunosorbent Assay
Hepacivirus / genetics,  immunology*
Hepatitis B / complications,  virology
Hepatitis B Antibodies / blood
Hepatitis B Core Antigens / analysis
Hepatitis B Surface Antigens / analysis
Hepatitis C / immunology,  virology*
Hepatitis C Antibodies / blood*
Liver Cirrhosis / immunology,  virology*
Liver Function Tests
Middle Aged
RNA, Viral / analysis*
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/DNA Primers; 0/Hepatitis B Antibodies; 0/Hepatitis B Core Antigens; 0/Hepatitis B Surface Antigens; 0/Hepatitis C Antibodies; 0/RNA, Viral

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