Document Detail

HCO3- secretion in mitochondria-rich cells is linked to an H+-ATPase.
MedLine Citation:
PMID:  2524169     Owner:  NLM     Status:  MEDLINE    
Turtle bladder mitochondria-rich (MR) cells secrete H+ by an ATP-dependent process. MR cells also secrete HCO3- by an energy-requiring, Cl- -dependent process that may depend on a serosal H+-ATPase. To determine whether HCO3- is linked to an H+-ATPase, O2 consumption was assessed in MR and granular (G) cells exposed to H+ and HCO3- transport inhibitors alone and also with an H+-ATPase inhibitor. MR and G cells were separated by Ficoll density-gradient centrifugation and treated with ouabain and 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid (SITS) to maximally inhibit Na+ and luminal H+ transport. O2 consumption was measured before and after cells were additionally treated with 10 microM N-ethylmaleimide (NEM), an inhibitor of nonmitochondrial H+-ATPase. O2 consumption of MR cells fell after treatment with NEM (delta = 8.64 +/- 2.35 microliters protein-1, P less than 0.025, n = 5). There was no significant difference in G cells similarly treated (delta = 1.61 +/- 0.62 microliters protein-1, P greater than 0.05, n = 5). Because luminal H+ secretion is nearly abolished after treatment with SITS, the decline in O2 consumption of 44.4 +/- 7.11% after addition of NEM is probably due to inhibition of other non-mitochondrial H+-ATPases. In the intact bladder, HCO3- secretion was reduced by 35.1% after serosal application of NEM. Furthermore, in SITS-treated MR and G cells, ATP levels as measured by the luciferin-luciferase assay method were not appreciably different in the presence or absence of NEM.(ABSTRACT TRUNCATED AT 250 WORDS)
C Fritsche; J H Schwartz; R R Heinen; R Tietz; J G Kleinman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  256     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1989 May 
Date Detail:
Created Date:  1989-06-16     Completed Date:  1989-06-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  F869-74     Citation Subset:  IM    
Department of Medicine, C. J. Zablocki Veterans Administration Medical Center, Milwaukee, Wisconsin.
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MeSH Terms
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / pharmacology
Adenosine Triphosphate / metabolism
Bicarbonates / secretion*
Biological Transport / drug effects
Ethylmaleimide / pharmacology
Granulocytes / metabolism
Hydrogen / metabolism
Mitochondria / enzymology,  metabolism,  secretion*
Oxygen Consumption / drug effects
Proton-Translocating ATPases / metabolism*
Urinary Bladder / cytology,  enzymology,  metabolism*
Reg. No./Substance:
0/Bicarbonates; 128-53-0/Ethylmaleimide; 1333-74-0/Hydrogen; 27816-59-7/4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; 56-65-5/Adenosine Triphosphate; EC ATPases

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