| HCG + ACTH stimulation of in vitro dehydroepiandrosterone production in human fetal adrenals from precursor cholesterol and delta5-pregnenolone. | |
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MedLine Citation:
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PMID: 178853 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fetal adrenal glands were obtained from legal abortions in the 14--22 gestational weeks. The adrenal cortex was separated from the medulla using a micromanipulator. The cortex was homogenized in a glass-teflon homogenizer and the microsomal fraction was isolated by centrifugation. Tissue slices were also prepared by the method of Deutsch. The microsomal fraction or the slices were incubated with (4-14C) pregnenolone or (4-14C) cholesterol in the presence of an NADPH regenerating system and oxygen. ACTH or HCG was added. After extraction and paperchromatography radioactivity was determined in a scanner. The conversion of pregnenolone to 17 alpha-hydroxypregnenolone and dehydroepiandrosterone was increased by ACTH. In the presence of HCG dehydroepiandrosterone production only was increased,--and this occurred in slices only. In the microsomal fraction HCG was without effect on steroid biogenesis from dehydroepiandrosterone. |
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Authors:
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W D Lehmann; C Lauritzen |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Journal of perinatal medicine Volume: 3 ISSN: 0300-5577 ISO Abbreviation: J Perinat Med Publication Date: 1975 |
Date Detail:
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Created Date: 1976-08-03 Completed Date: 1976-08-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0361031 Medline TA: J Perinat Med Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 231-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adrenal Cortex
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embryology*,
metabolism,
ultrastructure Adrenal Glands / embryology* Adrenocorticotropic Hormone / pharmacology* Cholesterol / metabolism Chorionic Gonadotropin / pharmacology* Dehydroepiandrosterone / biosynthesis* Gestational Age Humans Microsomes / metabolism Pregnenolone / metabolism Stimulation, Chemical |
| Chemical | |
Reg. No./Substance:
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0/Chorionic Gonadotropin; 145-13-1/Pregnenolone; 53-43-0/Dehydroepiandrosterone; 57-88-5/Cholesterol; 9002-60-2/Adrenocorticotropic Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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