Document Detail


HCG + ACTH stimulation of in vitro dehydroepiandrosterone production in human fetal adrenals from precursor cholesterol and delta5-pregnenolone.
MedLine Citation:
PMID:  178853     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fetal adrenal glands were obtained from legal abortions in the 14--22 gestational weeks. The adrenal cortex was separated from the medulla using a micromanipulator. The cortex was homogenized in a glass-teflon homogenizer and the microsomal fraction was isolated by centrifugation. Tissue slices were also prepared by the method of Deutsch. The microsomal fraction or the slices were incubated with (4-14C) pregnenolone or (4-14C) cholesterol in the presence of an NADPH regenerating system and oxygen. ACTH or HCG was added. After extraction and paperchromatography radioactivity was determined in a scanner. The conversion of pregnenolone to 17 alpha-hydroxypregnenolone and dehydroepiandrosterone was increased by ACTH. In the presence of HCG dehydroepiandrosterone production only was increased,--and this occurred in slices only. In the microsomal fraction HCG was without effect on steroid biogenesis from dehydroepiandrosterone.
Authors:
W D Lehmann; C Lauritzen
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Journal of perinatal medicine     Volume:  3     ISSN:  0300-5577     ISO Abbreviation:  J Perinat Med     Publication Date:  1975  
Date Detail:
Created Date:  1976-08-03     Completed Date:  1976-08-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0361031     Medline TA:  J Perinat Med     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  231-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adrenal Cortex / embryology*,  metabolism,  ultrastructure
Adrenal Glands / embryology*
Adrenocorticotropic Hormone / pharmacology*
Cholesterol / metabolism
Chorionic Gonadotropin / pharmacology*
Dehydroepiandrosterone / biosynthesis*
Gestational Age
Humans
Microsomes / metabolism
Pregnenolone / metabolism
Stimulation, Chemical
Chemical
Reg. No./Substance:
0/Chorionic Gonadotropin; 145-13-1/Pregnenolone; 53-43-0/Dehydroepiandrosterone; 57-88-5/Cholesterol; 9002-60-2/Adrenocorticotropic Hormone

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