Document Detail

HCF-1 self-association via an interdigitated Fn3 structure facilitates transcriptional regulatory complex formation.
MedLine Citation:
PMID:  23045687     Owner:  NLM     Status:  MEDLINE    
Host-cell factor 1 (HCF-1) is an unusual transcriptional regulator that undergoes a process of proteolytic maturation to generate N- (HCF-1(N)) and C- (HCF-1(C)) terminal subunits noncovalently associated via self-association sequence elements. Here, we present the crystal structure of the self-association sequence 1 (SAS1) including the adjacent C-terminal HCF-1 nuclear localization signal (NLS). SAS1 elements from each of the HCF-1(N) and HCF-1(C) subunits form an interdigitated fibronectin type 3 (Fn3) tandem repeat structure. We show that the C-terminal NLS recruited by the interdigitated SAS1 structure is required for effective formation of a transcriptional regulatory complex: the herpes simplex virus VP16-induced complex. Thus, HCF-1(N)-HCF-1(C) association via an integrated Fn3 structure permits an NLS to facilitate formation of a transcriptional regulatory complex.
Jihye Park; Fabienne Lammers; Winship Herr; Ji-Joon Song
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-08
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  109     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-24     Completed Date:  2013-01-07     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  17430-5     Citation Subset:  IM    
Department of Biological Sciences and Graduate School of Nanoscience and Technology, World Class University, Korea Advanced Institute of Science and Technology, Institute for the BioCentury, KAIST, Daejeon 305-701, Korea.
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MeSH Terms
Amino Acid Sequence
Crystallography, X-Ray
Gene Expression Regulation*
Host Cell Factor C1 / chemistry,  physiology*
Models, Molecular
Molecular Sequence Data
Nuclear Localization Signals
Tandem Repeat Sequences
Transcription, Genetic*
Reg. No./Substance:
0/HCFC1 protein, human; 0/Host Cell Factor C1; 0/Nuclear Localization Signals

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