| HAS3-related hyaluronan enhances biological activities necessary for metastasis of osteosarcoma cells. | |
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MedLine Citation:
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PMID: 16773198 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Several studies have suggested that increased production of hyaluronan (HA) is associated with metastatic behavior in various malignant tumors. To our knowledge, HA molecular weights required for metastasis are still unsolved in osteosarcoma. We examined the size of HA and hyaluronan synthase (HAS) isoforms related to biological functions required for metastasis in the LM8 stably highly metastatic osteosarcoma cell line. We found that HA of molecular weight which HAS3 produces enhanced biological functions related to metastasis such as cell proliferation, invasion, and degradation of extracellular matrix. Moreover, cell proliferation and invasion were inhibited by suppressing the activity of HAS3 expressed in LM8 cells, using hyaluronan synthase suppressor, 4-methylumbelliferone (MU). HA with the molecular weight related to HAS2 was the most adherent to CD44 in LM8 cells, suggesting that HAS2 may play an important role in pericellular coat formation. These results suggest that HAS3-related HA enhances crucial biological activities necessary for metastasis and that HAS2-related HA offers an advantageous environment for osteosarcoma cells. |
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Authors:
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Katsuhiro Tofuku; Masahiro Yokouchi; Takashi Murayama; Shusaku Minami; Setsuro Komiya |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: International journal of oncology Volume: 29 ISSN: 1019-6439 ISO Abbreviation: Int. J. Oncol. Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-06-14 Completed Date: 2007-08-03 Revised Date: 2012-06-05 |
Medline Journal Info:
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Nlm Unique ID: 9306042 Medline TA: Int J Oncol Country: Greece |
Other Details:
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Languages: eng Pagination: 175-83 Citation Subset: IM |
Affiliation:
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Department of Orthopaedic Surgery, Kagoshima Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD44 / genetics, metabolism Cell Adhesion / drug effects Cell Line, Tumor Cell Movement / drug effects Cell Proliferation / drug effects Collagen Dose-Response Relationship, Drug Drug Combinations Enzyme Inhibitors / pharmacology Extracellular Signal-Regulated MAP Kinases / metabolism Focal Adhesion Kinase 1 / metabolism Gene Expression Regulation, Neoplastic* Glucuronosyltransferase / antagonists & inhibitors, genetics, metabolism* Humans Hyaluronic Acid / chemistry, pharmacology* Hymecromone / analogs & derivatives, pharmacology Laminin Matrix Metalloproteinase 2 / metabolism Mice Molecular Weight Neoplasm Invasiveness Neoplasm Metastasis Osteosarcoma / enzymology*, genetics, pathology Phosphorylation Proteoglycans Proto-Oncogene Proteins c-fos / genetics, metabolism RNA, Messenger / metabolism |
| Chemical | |
Reg. No./Substance:
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0/4-methylumbelliferone; 0/Antigens, CD44; 0/CD44 protein, human; 0/Drug Combinations; 0/Enzyme Inhibitors; 0/Laminin; 0/Proteoglycans; 0/Proto-Oncogene Proteins c-fos; 0/RNA, Messenger; 119978-18-6/matrigel; 90-33-5/Hymecromone; 9004-61-9/Hyaluronic Acid; 9007-34-5/Collagen; EC 2.4.1.-/HAS2 protein, human; EC 2.4.1.-/HAS3 protein, human; EC 2.4.1.17/Glucuronosyltransferase; EC 2.7.10.1/Focal Adhesion Kinase 1; EC 2.7.10.2/Ptk2 protein, mouse; EC 2.7.11.24/Extracellular Signal-Regulated MAP Kinases; EC 3.4.24.24/Matrix Metalloproteinase 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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