Document Detail


HAS3-related hyaluronan enhances biological activities necessary for metastasis of osteosarcoma cells.
MedLine Citation:
PMID:  16773198     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several studies have suggested that increased production of hyaluronan (HA) is associated with metastatic behavior in various malignant tumors. To our knowledge, HA molecular weights required for metastasis are still unsolved in osteosarcoma. We examined the size of HA and hyaluronan synthase (HAS) isoforms related to biological functions required for metastasis in the LM8 stably highly metastatic osteosarcoma cell line. We found that HA of molecular weight which HAS3 produces enhanced biological functions related to metastasis such as cell proliferation, invasion, and degradation of extracellular matrix. Moreover, cell proliferation and invasion were inhibited by suppressing the activity of HAS3 expressed in LM8 cells, using hyaluronan synthase suppressor, 4-methylumbelliferone (MU). HA with the molecular weight related to HAS2 was the most adherent to CD44 in LM8 cells, suggesting that HAS2 may play an important role in pericellular coat formation. These results suggest that HAS3-related HA enhances crucial biological activities necessary for metastasis and that HAS2-related HA offers an advantageous environment for osteosarcoma cells.
Authors:
Katsuhiro Tofuku; Masahiro Yokouchi; Takashi Murayama; Shusaku Minami; Setsuro Komiya
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  International journal of oncology     Volume:  29     ISSN:  1019-6439     ISO Abbreviation:  Int. J. Oncol.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-06-14     Completed Date:  2007-08-03     Revised Date:  2012-06-05    
Medline Journal Info:
Nlm Unique ID:  9306042     Medline TA:  Int J Oncol     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  175-83     Citation Subset:  IM    
Affiliation:
Department of Orthopaedic Surgery, Kagoshima Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD44 / genetics,  metabolism
Cell Adhesion / drug effects
Cell Line, Tumor
Cell Movement / drug effects
Cell Proliferation / drug effects
Collagen
Dose-Response Relationship, Drug
Drug Combinations
Enzyme Inhibitors / pharmacology
Extracellular Signal-Regulated MAP Kinases / metabolism
Focal Adhesion Kinase 1 / metabolism
Gene Expression Regulation, Neoplastic*
Glucuronosyltransferase / antagonists & inhibitors,  genetics,  metabolism*
Humans
Hyaluronic Acid / chemistry,  pharmacology*
Hymecromone / analogs & derivatives,  pharmacology
Laminin
Matrix Metalloproteinase 2 / metabolism
Mice
Molecular Weight
Neoplasm Invasiveness
Neoplasm Metastasis
Osteosarcoma / enzymology*,  genetics,  pathology
Phosphorylation
Proteoglycans
Proto-Oncogene Proteins c-fos / genetics,  metabolism
RNA, Messenger / metabolism
Chemical
Reg. No./Substance:
0/4-methylumbelliferone; 0/Antigens, CD44; 0/CD44 protein, human; 0/Drug Combinations; 0/Enzyme Inhibitors; 0/Laminin; 0/Proteoglycans; 0/Proto-Oncogene Proteins c-fos; 0/RNA, Messenger; 119978-18-6/matrigel; 90-33-5/Hymecromone; 9004-61-9/Hyaluronic Acid; 9007-34-5/Collagen; EC 2.4.1.-/HAS2 protein, human; EC 2.4.1.-/HAS3 protein, human; EC 2.4.1.17/Glucuronosyltransferase; EC 2.7.10.1/Focal Adhesion Kinase 1; EC 2.7.10.2/Ptk2 protein, mouse; EC 2.7.11.24/Extracellular Signal-Regulated MAP Kinases; EC 3.4.24.24/Matrix Metalloproteinase 2

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