| A HAPPY map of Cryptosporidium parvum. | |
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MedLine Citation:
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PMID: 9872984 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have constructed a HAPPY map of the apicomplexan parasite Cryptosporidium parvum. We have placed 204 markers on the 10.4-Mb genome, giving an average marker spacing of approximately 50 kb, with an effective resolution of approximately 40 kb. HAPPY mapping (an in vitro linkage technique based on screening approximately haploid amounts of DNA by the polymerase chain reaction) is fast and accurate and is not subject to the distortions inherent in cloning, meiotic recombination, or hybrid cell formation. In addition, little genomic DNA is needed as a substrate, and the AT content of the genome is largely immaterial, making it an ideal method for mapping otherwise intractable parasite genomes. The map, covering all eight chromosomes, consists of 10 linkage groups, each of which has been chromosomally assigned. We have verified the accuracy of the map by several methods, including the construction of a >140-kb PAC contig on chromosome VI. Less than 1% of our markers detect non-rDNA duplicated sequences. |
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Authors:
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M B Piper; A T Bankier; P H Dear |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Genome research Volume: 8 ISSN: 1088-9051 ISO Abbreviation: Genome Res. Publication Date: 1998 Dec |
Date Detail:
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Created Date: 1999-06-25 Completed Date: 1999-06-25 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 9518021 Medline TA: Genome Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1299-307 Citation Subset: IM |
Affiliation:
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Medical Research Council Laboratory of Molecular Biology, Protein and Nucleic Acid Chemistry Division, Cambridge CB2 2QH, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Southern Chromosome Banding Chromosome Mapping / methods* Contig Mapping Cryptosporidium parvum / genetics* DNA, Protozoan / analysis Genetic Linkage Genetic Markers |
| Chemical | |
Reg. No./Substance:
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0/DNA, Protozoan; 0/Genetic Markers |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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