| H2O2 at physiological concentrations modulates Leydig cell function inducing oxidative stress and apoptosis. | |
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MedLine Citation:
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PMID: 16374549 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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H(2)O(2) is one of the active reactive oxygen species secreted by macrophages that are seen closely aligned with Leydig cells in the testicular interstitium. The present study was initiated to investigate the role of H(2)O(2) on Leydig cell function in vitro at physiological concentrations. Significant decrease in both testosterone production (p < 0.05) and 3 beta-hydroxysteroid dehydrogenase activity (p < 0.05) in adult Leydig cells were observed even with H(2)O(2) at low concentrations (30 - 50 microM). H(2)O(2) exposure increased oxidative stress in Leydig cells with the rise in lipid peroxidation and fall in the activities of the antioxidant enzymes; superoxide dismutase (SOD), catalase (CAT) & glutathione-s-transferase (GST). There was also a marginal increase (approximately 8%) in cell apoptosis accompanied by rise in FasL expression and caspase-3 activation. The above findings indicate that H(2)O(2) as a bio-molecule modulates Leydig cell function at or below physiological concentrations through a variety of actions like decrease in steroidogenic enzyme activity and increase in oxidative stress and apoptosis. |
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Authors:
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D K Gautam; M M Misro; S P Chaki; N Sehgal |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Apoptosis : an international journal on programmed cell death Volume: 11 ISSN: 1360-8185 ISO Abbreviation: Apoptosis Publication Date: 2006 Jan |
Date Detail:
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Created Date: 2006-01-26 Completed Date: 2007-05-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9712129 Medline TA: Apoptosis Country: United States |
Other Details:
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Languages: eng Pagination: 39-46 Citation Subset: IM |
Affiliation:
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Department of Reproductive Biomedicine, National Institute of Health and Family Welfare, New Mehrauli Road, Munirka, New Delhi. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3-Hydroxysteroid Dehydrogenases
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metabolism Animals Apoptosis / drug effects*, physiology Caspase 3 / metabolism Catalase / metabolism Fas Ligand Protein / metabolism Glutathione Transferase / metabolism Hydrogen Peroxide / metabolism, pharmacology* Leydig Cells / cytology*, drug effects*, metabolism Lipid Peroxidation / drug effects Male Oxidative Stress / drug effects Rats Rats, Sprague-Dawley Superoxide Dismutase / metabolism Testosterone / biosynthesis |
| Chemical | |
Reg. No./Substance:
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0/Fas Ligand Protein; 0/Tnfsf6 protein, rat; 58-22-0/Testosterone; 7722-84-1/Hydrogen Peroxide; EC 1.1.-/3-Hydroxysteroid Dehydrogenases; EC 1.11.1.6/Catalase; EC 1.15.1.1/Superoxide Dismutase; EC 2.5.1.18/Glutathione Transferase; EC 3.4.22.-/Casp3 protein, rat; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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