Document Detail

H2O2 at physiological concentrations modulates Leydig cell function inducing oxidative stress and apoptosis.
MedLine Citation:
PMID:  16374549     Owner:  NLM     Status:  MEDLINE    
H(2)O(2) is one of the active reactive oxygen species secreted by macrophages that are seen closely aligned with Leydig cells in the testicular interstitium. The present study was initiated to investigate the role of H(2)O(2) on Leydig cell function in vitro at physiological concentrations. Significant decrease in both testosterone production (p < 0.05) and 3 beta-hydroxysteroid dehydrogenase activity (p < 0.05) in adult Leydig cells were observed even with H(2)O(2) at low concentrations (30 - 50 microM). H(2)O(2) exposure increased oxidative stress in Leydig cells with the rise in lipid peroxidation and fall in the activities of the antioxidant enzymes; superoxide dismutase (SOD), catalase (CAT) & glutathione-s-transferase (GST). There was also a marginal increase (approximately 8%) in cell apoptosis accompanied by rise in FasL expression and caspase-3 activation. The above findings indicate that H(2)O(2) as a bio-molecule modulates Leydig cell function at or below physiological concentrations through a variety of actions like decrease in steroidogenic enzyme activity and increase in oxidative stress and apoptosis.
D K Gautam; M M Misro; S P Chaki; N Sehgal
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Apoptosis : an international journal on programmed cell death     Volume:  11     ISSN:  1360-8185     ISO Abbreviation:  Apoptosis     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-01-26     Completed Date:  2007-05-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9712129     Medline TA:  Apoptosis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  39-46     Citation Subset:  IM    
Department of Reproductive Biomedicine, National Institute of Health and Family Welfare, New Mehrauli Road, Munirka, New Delhi.
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MeSH Terms
3-Hydroxysteroid Dehydrogenases / metabolism
Apoptosis / drug effects*,  physiology
Caspase 3 / metabolism
Catalase / metabolism
Fas Ligand Protein / metabolism
Glutathione Transferase / metabolism
Hydrogen Peroxide / metabolism,  pharmacology*
Leydig Cells / cytology*,  drug effects*,  metabolism
Lipid Peroxidation / drug effects
Oxidative Stress / drug effects
Rats, Sprague-Dawley
Superoxide Dismutase / metabolism
Testosterone / biosynthesis
Reg. No./Substance:
0/Fas Ligand Protein; 0/Tnfsf6 protein, rat; 58-22-0/Testosterone; 7722-84-1/Hydrogen Peroxide; EC 1.1.-/3-Hydroxysteroid Dehydrogenases; EC; EC Dismutase; EC Transferase; EC 3.4.22.-/Casp3 protein, rat; EC 3.4.22.-/Caspase 3

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