Document Detail


H2O2 at physiological concentrations modulates Leydig cell function inducing oxidative stress and apoptosis.
MedLine Citation:
PMID:  16374549     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
H(2)O(2) is one of the active reactive oxygen species secreted by macrophages that are seen closely aligned with Leydig cells in the testicular interstitium. The present study was initiated to investigate the role of H(2)O(2) on Leydig cell function in vitro at physiological concentrations. Significant decrease in both testosterone production (p < 0.05) and 3 beta-hydroxysteroid dehydrogenase activity (p < 0.05) in adult Leydig cells were observed even with H(2)O(2) at low concentrations (30 - 50 microM). H(2)O(2) exposure increased oxidative stress in Leydig cells with the rise in lipid peroxidation and fall in the activities of the antioxidant enzymes; superoxide dismutase (SOD), catalase (CAT) & glutathione-s-transferase (GST). There was also a marginal increase (approximately 8%) in cell apoptosis accompanied by rise in FasL expression and caspase-3 activation. The above findings indicate that H(2)O(2) as a bio-molecule modulates Leydig cell function at or below physiological concentrations through a variety of actions like decrease in steroidogenic enzyme activity and increase in oxidative stress and apoptosis.
Authors:
D K Gautam; M M Misro; S P Chaki; N Sehgal
Related Documents :
22216309 - The cytokine release inhibitory drug crid3 targets asc oligomerisation in the nlrp3 and...
23404369 - The role of mast cells in neuroinflammation.
23798999 - Interleukin-22 mediates early host defense against rhizomucor pusilluscan pathogens.
24252829 - The immune response against candida spp. and sporothrix schenckii.
23766529 - Kupffer cells potentiate liver sinusoidal endothelial cell injury in sepsis by ligating...
23278739 - The function of sub-synaptic vesicles during t-cell activation.
17986489 - The role of innate immunity in the pathogenesis of asthma: evidence for the involvement...
12570669 - Intestinal epithelial toll-like receptors: to protect. and serve?
12447609 - Macrophage chemotactic protein-1 and macrophage inflammatory protein-1 alpha induce nit...
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Apoptosis : an international journal on programmed cell death     Volume:  11     ISSN:  1360-8185     ISO Abbreviation:  Apoptosis     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-01-26     Completed Date:  2007-05-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9712129     Medline TA:  Apoptosis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  39-46     Citation Subset:  IM    
Affiliation:
Department of Reproductive Biomedicine, National Institute of Health and Family Welfare, New Mehrauli Road, Munirka, New Delhi.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
3-Hydroxysteroid Dehydrogenases / metabolism
Animals
Apoptosis / drug effects*,  physiology
Caspase 3 / metabolism
Catalase / metabolism
Fas Ligand Protein / metabolism
Glutathione Transferase / metabolism
Hydrogen Peroxide / metabolism,  pharmacology*
Leydig Cells / cytology*,  drug effects*,  metabolism
Lipid Peroxidation / drug effects
Male
Oxidative Stress / drug effects
Rats
Rats, Sprague-Dawley
Superoxide Dismutase / metabolism
Testosterone / biosynthesis
Chemical
Reg. No./Substance:
0/Fas Ligand Protein; 0/Tnfsf6 protein, rat; 58-22-0/Testosterone; 7722-84-1/Hydrogen Peroxide; EC 1.1.-/3-Hydroxysteroid Dehydrogenases; EC 1.11.1.6/Catalase; EC 1.15.1.1/Superoxide Dismutase; EC 2.5.1.18/Glutathione Transferase; EC 3.4.22.-/Casp3 protein, rat; EC 3.4.22.-/Caspase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Role of the unfolded protein response in cell death.
Next Document:  Protection of human and murine hepatocytes against Fas-induced death by transferrin and iron.