Document Detail

H-Ras, R-Ras, and TC21 differentially regulate ureteric bud cell branching morphogenesis.
MedLine Citation:
PMID:  16467383     Owner:  NLM     Status:  MEDLINE    
The collecting system of the kidney, derived from the ureteric bud (UB), undergoes repetitive bifid branching events during early development followed by a phase of tubular growth and elongation. Although members of the Ras GTPase family control cell growth, differentiation, proliferation, and migration, their role in development of the collecting system of the kidney is unexplored. In this study, we demonstrate that members of the R-Ras family of proteins, R-Ras and TC21, are expressed in the murine collecting system at E13.5, whereas H-Ras is only detected at day E17.5. Using murine UB cells expressing activated H-Ras, R-Ras, and TC21, we demonstrate that R-Ras-expressing cells show increased branching morphogenesis and cell growth, TC21-expressing cells branch excessively but lose their ability to migrate, whereas H-Ras-expressing cells migrated the most and formed long unbranched tubules. These differences in branching morphogenesis are mediated by differential regulation/activation of the Rho family of GTPases and mitogen-activated protein kinases. Because most branching of the UB occurs early in development, it is conceivable that R-Ras and TC-21 play a role in facilitating branching and growth in early UB development, whereas H-Ras might favor cell migration and elongation of tubules, events that occur later in development.
Ambra Pozzi; Sergio Coffa; Nada Bulus; Wenqin Zhu; Dong Chen; Xiwu Chen; Glenda Mernaugh; Yan Su; Songmin Cai; Amar Singh; Marcela Brissova; Roy Zent
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2006-02-08
Journal Detail:
Title:  Molecular biology of the cell     Volume:  17     ISSN:  1059-1524     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-03-29     Completed Date:  2006-06-28     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2046-56     Citation Subset:  IM    
Department of Research Medicine, Veterans Affairs Hospital, Nashville, TN 37232, USA.
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MeSH Terms
Cell Movement
Cell Proliferation
Cells, Cultured
Enzyme Activation
Epithelium / embryology,  enzymology
Extracellular Signal-Regulated MAP Kinases / antagonists & inhibitors,  metabolism
Kidney Tubules, Collecting / chemistry,  embryology*,  enzymology
Membrane Proteins / analysis,  genetics,  physiology*
Mesoderm / enzymology
Monomeric GTP-Binding Proteins / analysis,  genetics,  metabolism,  physiology*
Signal Transduction
Ureter / chemistry,  embryology*,  enzymology
p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors,  metabolism
ras Proteins / analysis,  genetics,  physiology*
Grant Support
Reg. No./Substance:
0/Membrane Proteins; EC Signal-Regulated MAP Kinases; EC Mitogen-Activated Protein Kinases; EC 3.6.1/Rras2 protein, mouse; EC GTP-Binding Proteins; EC Proteins

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