| H-Ras, R-Ras, and TC21 differentially regulate ureteric bud cell branching morphogenesis. | |
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MedLine Citation:
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PMID: 16467383 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The collecting system of the kidney, derived from the ureteric bud (UB), undergoes repetitive bifid branching events during early development followed by a phase of tubular growth and elongation. Although members of the Ras GTPase family control cell growth, differentiation, proliferation, and migration, their role in development of the collecting system of the kidney is unexplored. In this study, we demonstrate that members of the R-Ras family of proteins, R-Ras and TC21, are expressed in the murine collecting system at E13.5, whereas H-Ras is only detected at day E17.5. Using murine UB cells expressing activated H-Ras, R-Ras, and TC21, we demonstrate that R-Ras-expressing cells show increased branching morphogenesis and cell growth, TC21-expressing cells branch excessively but lose their ability to migrate, whereas H-Ras-expressing cells migrated the most and formed long unbranched tubules. These differences in branching morphogenesis are mediated by differential regulation/activation of the Rho family of GTPases and mitogen-activated protein kinases. Because most branching of the UB occurs early in development, it is conceivable that R-Ras and TC-21 play a role in facilitating branching and growth in early UB development, whereas H-Ras might favor cell migration and elongation of tubules, events that occur later in development. |
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Authors:
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Ambra Pozzi; Sergio Coffa; Nada Bulus; Wenqin Zhu; Dong Chen; Xiwu Chen; Glenda Mernaugh; Yan Su; Songmin Cai; Amar Singh; Marcela Brissova; Roy Zent |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2006-02-08 |
Journal Detail:
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Title: Molecular biology of the cell Volume: 17 ISSN: 1059-1524 ISO Abbreviation: Mol. Biol. Cell Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-03-29 Completed Date: 2006-06-28 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: United States |
Other Details:
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Languages: eng Pagination: 2046-56 Citation Subset: IM |
Affiliation:
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Department of Research Medicine, Veterans Affairs Hospital, Nashville, TN 37232, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Movement Cell Proliferation Cells, Cultured Enzyme Activation Epithelium / embryology, enzymology Extracellular Signal-Regulated MAP Kinases / antagonists & inhibitors, metabolism Kidney Tubules, Collecting / chemistry, embryology*, enzymology Membrane Proteins / analysis, genetics, physiology* Mesoderm / enzymology Mice Monomeric GTP-Binding Proteins / analysis, genetics, metabolism, physiology* Morphogenesis* Signal Transduction Ureter / chemistry, embryology*, enzymology p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors, metabolism ras Proteins / analysis, genetics, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA 94849/CA/NCI NIH HHS; R01 DK 074359/DK/NIDDK NIH HHS; R01 DK 69921/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Membrane Proteins; EC 2.7.11.24/Extracellular Signal-Regulated MAP Kinases; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases; EC 3.6.1/Rras2 protein, mouse; EC 3.6.5.2/Monomeric GTP-Binding Proteins; EC 3.6.5.2/ras Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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