Document Detail

An H-2K gene of the tw32 mutant at the T/t complex is a close parent of an H-2Kq gene.
MedLine Citation:
PMID:  3838967     Owner:  NLM     Status:  MEDLINE    
Two recombinant mice have been recovered from the progeny of Ttf/tw32 + animals. They have lost the tw32 lethality factor(s) and gained tufted, presumably from the T chromosome. Southern blot analysis of class I genes of these two new partial tPA027 and tPA286 haplotypes indicates that they have retained at least part of the major histocompatibility complex of the tw32 chromosome (H-2 haplotype H-2w28). We have prepared a phage library of Eco RI-digested DNA from homozygous tPA027 animals. Upon screening the library with a cDNA probe specific for H-2K genes, we isolated a class I gene displaying all of the distinctive features of a genuine H-2K gene, and which could thus be defined as an H-2Kw28 gene. The H-2Kw28 gene is 92-95% homologous to H-2Kb and H-2Kd genes and differs significantly from the other class I genes sequenced so far. Homology with the H-2Kb sequence reaches nearly 100% in the 3' part of the H-2Kw28 gene. Moreover, the homology with an H-2Kq cDNA sequence reaches 99.8%. Several hypotheses can account for the near identity of H-2Kb, H-2Kq, and H-2Kw28 gene sequences: either recombination between H-2w28 and H-2b and H-2q sequences occurred before or at the time the strain was established, or the class I genes of the tw32 chromosome and the H-2b and H-2q genes found in inbred strains of mice have separated from each other rather recently.
T Morita; C Delarbre; M Kress; P Kourilsky; G Gachelin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunogenetics     Volume:  21     ISSN:  0093-7711     ISO Abbreviation:  Immunogenetics     Publication Date:  1985  
Date Detail:
Created Date:  1985-07-09     Completed Date:  1985-07-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0420404     Medline TA:  Immunogenetics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  367-83     Citation Subset:  IM    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M14825;  M14826;  M14827
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MeSH Terms
Amino Acid Sequence
Base Sequence
Chromosome Mapping
Cloning, Molecular
DNA / genetics
H-2 Antigens / genetics*
Recombination, Genetic
Reg. No./Substance:
0/H-2 Antigens; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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