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Gut Fermentation Products of Inulin-type Fructans Modulate the Expression of Xenobiotic-metabolising Enzymes in Human Colonic Tumour Cells.
MedLine Citation:
PMID:  23225440     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Epidemiological studies suggest that nutrition plays an important role in colonic cancer prevention. A possible mechanism of this prevention may be the modulation of carcinogen metabolism and scavenging of reactive intermediates. In particular, dietary fibres are discussed as potentially protective food ingredients. Therefore, the aim of this study was to analyse if the expression of genes related to biotransformation is modulated by fermentation samples of dietary fibres. HT29 (human colonic carcinoma) and LT97 (human adenoma) cells were incubated with fermentation supernatant (SFS), produced by in vitro fermentation of inulin enriched with oligofructose. Possible mechanisms of de-toxification were investigated by analysing the expression of catalase and glutathione-S-transferase (GST) A4 mRNA and the enzyme activity of catalase and GST. In addition, protection of both cell lines against DNA damage, induced by H(2)O(2) or 4-hydroxynonenal (HNE), was analysed using the comet assay. Incubation with SFS resulted in significantly increased GSTA4 mRNA expression, significantly enhanced catalase activity and a significant reduction in the amount of H(2)O(2)-induced DNA damage in HT29 cells. Our results show that complex fermentation samples of inulin-type fructans favourably modulate expression of genes related to biotransformation in carcinoma cells, which in turn supports the important role of dietary fibres in primary chemoprevention.
Authors:
Umang Munjal; Daniel Scharlau; Michael Glei
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Anticancer research     Volume:  32     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  5379-86     Citation Subset:  IM    
Affiliation:
Friedrich-Schiller-University Jena, Biological-Pharmaceutical Faculty, Institute for Nutrition, Department of Nutritional Toxicology, Dornburger Str. 24, 07743 Jena, Germany. umangmiglani@hotmail.com.
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