Document Detail

Guided tissue regeneration may modulate gene expression in periodontal intrabony defects: a human study.
MedLine Citation:
PMID:  18503515     Owner:  NLM     Status:  MEDLINE    
BACKGROUND AND OBJECTIVE: Guided tissue regeneration has been shown to lead to periodontal regeneration, however, the mechanisms involved remain to be clarified. The present study was carried out to assess the expression of genes involved in the healing process of periodontal tissues in membrane-protected vs. nonprotected intrabony defects in humans. MATERIAL AND METHODS: Thirty patients with deep intrabony defects (> or = 5 mm, two or three walls) around teeth that were scheduled for extraction were selected and randomly assigned to receive one of the following treatments: flap surgery alone (control group) or flap surgery plus guided tissue regeneration (expanded polytetrafluorethylene (e-PTFE) membrane) (test group). Twenty-one days later, the newly formed tissue was harvested and quantitatively assessed using the polymerase chain reaction assay for the expression of the following genes: alkaline phosphatase, receptor activator of nuclear factor-kappa B ligand, osteoprotegerin, osteopontin, osteocalcin, bone sialoprotein, basic fibroblast growth factor, interleukin-1, interleukin-4, interleukin-6, matrix metalloproteinase-2 and matrix metalloproteinase-9. RESULTS: Data analysis demonstrated that mRNA levels for alkaline phosphatase, receptor activator of nuclear factor-kappa B ligand, osteoprotegerin, osteopontin, bone sialoprotein, basic fibroblast growth factor, interleukin-1, interleukin-6, matrix metalloproteinase-2 and matrix metalloproteinase -9 were higher in the sites where guided tissue regeneration was applied compared with the control sites (p < 0.05), whereas osteocalcin mRNA levels were lower (p < 0.05). No difference was observed in interleukin-4 mRNA levels between control and test groups. CONCLUSION: Within the limits of this study, it can be concluded that genes are differentially expressed in membrane barrier-led periodontal healing when compared with flap surgery alone, and this may account for the clinical outcome achieved by guided tissue regeneration.
L L Lima; P F Gonçalves; E A Sallum; M Z Casati; F H Nociti
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Publication Detail:
Type:  Comparative Study; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of periodontal research     Volume:  43     ISSN:  1600-0765     ISO Abbreviation:  J. Periodont. Res.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-10-22     Completed Date:  2008-11-18     Revised Date:  2010-02-04    
Medline Journal Info:
Nlm Unique ID:  0055107     Medline TA:  J Periodontal Res     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  459-64     Citation Subset:  D; IM    
Division of Periodontics, School of Dentistry at Piracicaba, University of Campinas, Piracicaba, SP, Brazil.
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MeSH Terms
Alkaline Phosphatase / analysis
Alveolar Bone Loss / genetics,  surgery*
Fibroblast Growth Factor 2 / analysis
Gene Expression Regulation
Gene Expression Regulation, Enzymologic
Guided Tissue Regeneration, Periodontal / methods*
Interleukin-1 / analysis
Interleukin-4 / analysis
Interleukin-6 / analysis
Matrix Metalloproteinase 2 / analysis
Matrix Metalloproteinase 9 / analysis
Membranes, Artificial
Osteocalcin / analysis
Osteopontin / analysis
Osteoprotegerin / analysis
Polymerase Chain Reaction
RANK Ligand / analysis
Sialoglycoproteins / analysis
Single-Blind Method
Surgical Flaps
Wound Healing / genetics
Reg. No./Substance:
0/Interleukin-1; 0/Interleukin-6; 0/Membranes, Artificial; 0/Osteoprotegerin; 0/RANK Ligand; 0/Sialoglycoproteins; 0/integrin-binding sialoprotein; 103107-01-3/Fibroblast Growth Factor 2; 104982-03-8/Osteocalcin; 106441-73-0/Osteopontin; 207137-56-2/Interleukin-4; 9002-84-0/Polytetrafluoroethylene; EC Phosphatase; EC Metalloproteinase 2; EC Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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