Document Detail

Guided orientation of cardiomyocytes on electrospun aligned nanofibers for cardiac tissue engineering.
MedLine Citation:
PMID:  21681953     Owner:  NLM     Status:  MEDLINE    
Cardiac tissue engineering (TE) is one of the most promising strategies to reconstruct the infarct myocardium and the major challenge involves producing a bioactive scaffold with anisotropic properties that assist in cell guidance to mimic the heart tissue. In this study, random and aligned poly(ε-caprolactone)/gelatin (PG) composite nanofibrous scaffolds were electrospun to structurally mimic the oriented extracellular matrix (ECM). Morphological, chemical and mechanical properties of the electrospun PG nanofibers were evaluated by scanning electron microscopy (SEM), water contact angle, attenuated total reflectance Fourier transform infrared spectroscopy and tensile measurements. Results indicated that PG nanofibrous scaffolds possessed smaller fiber diameters (239 ± 37 nm for random fibers and 269 ± 33 nm for aligned fibers), increased hydrophilicity, and lower stiffness compared to electrospun PCL nanofibers. The aligned PG nanofibers showed anisotropic wetting characteristics and mechanical properties, which closely match the requirements of native cardiac anisotropy. Rabbit cardiomyocytes were cultured on electrospun random and aligned nanofibers to assess the biocompatibility of scaffolds, together with its potential for cell guidance. The SEM and immunocytochemical analysis showed that the aligned PG scaffold greatly promoted cell attachment and alignment because of the biological components and ordered topography of the scaffolds. Moreover, we concluded that the aligned PG nanofibrous scaffolds could be more promising substrates suitable for the regeneration of infarct myocardium and other cardiac defects.
Dan Kai; Molamma P Prabhakaran; Guorui Jin; Seeram Ramakrishna
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-06-16
Journal Detail:
Title:  Journal of biomedical materials research. Part B, Applied biomaterials     Volume:  98     ISSN:  1552-4981     ISO Abbreviation:  J. Biomed. Mater. Res. Part B Appl. Biomater.     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-07-06     Completed Date:  2012-02-01     Revised Date:  2013-06-10    
Medline Journal Info:
Nlm Unique ID:  101234238     Medline TA:  J Biomed Mater Res B Appl Biomater     Country:  United States    
Other Details:
Languages:  eng     Pagination:  379-86     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley Periodicals, Inc.
NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore.
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MeSH Terms
Cell Adhesion
Cell Culture Techniques
Mechanical Phenomena
Myocardium / cytology
Myocytes, Cardiac / cytology*
Nanofibers / chemistry*,  therapeutic use
Tissue Engineering / methods*
Tissue Scaffolds / chemistry

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