Document Detail

Guanosine induces necrosis of cultured aortic endothelial cells.
MedLine Citation:
PMID:  8053499     Owner:  NLM     Status:  MEDLINE    
We have observed that treatment of cultured bovine aortic endothelial (BAE) cells with guanosine can inhibit the proliferation and viability of the cells. The addition of 500 mumol/L guanosine to the medium resulted in approximately 90% inhibition of cell proliferation. It also changed the morphology of BAE cells from having a small cobblestone-like appearance to a giant pancake-like morphology. At a concentration range of 1 to 2.5 mmol/L, guanosine inhibited the viability of quiescent BAE cells. Incubation of the cells with 2 mmol/L guanosine for 24 hours maximally induced the loss of cell viability by approximately 80%. We also compared the effects of different nucleosides on the proliferation and viability of BAE cells and found that at appropriate concentration ranges, only guanosine was able to inhibit the proliferation and viability of the cells. To assess the mechanism that mediates the cytotoxicity of guanosine, we analyzed the degradation pattern of DNA in guanosine-treated cells and found that random DNA degradation occurred in the cells. Thus, we suggest that treatment of BAE cells with guanosine induced cell necrosis.
Z Han; J H Wyche
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The American journal of pathology     Volume:  145     ISSN:  0002-9440     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  1994 Aug 
Date Detail:
Created Date:  1994-09-08     Completed Date:  1994-09-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  423-7     Citation Subset:  AIM; IM    
Section of Molecular, Cellular, and Development Biology, Brown University, Providence, Rhode Island 02912.
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MeSH Terms
Aorta / drug effects*,  metabolism,  pathology*
Cell Division / drug effects
Cell Survival / drug effects
Cells, Cultured
DNA / metabolism
Endothelium, Vascular / drug effects*,  metabolism,  pathology*
Guanosine / pharmacology*
Reg. No./Substance:
118-00-3/Guanosine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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