| Guanidine group specific ADP-ribosyltransferase in murine cells. | |
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MedLine Citation:
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PMID: 1902105 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have identified a guanidine group specific ADP-ribosyltransferase activity, capable of transferring an ADP-ribose group from NAD to a low molecular weight guanidine compound [p-(nitrobenzylidine)amino]guanidine and proteins such as histone and poly-L-arginine, in a variety of murine cell lines. The enzyme activity appears to be associated with an integral membrane protein of apparent molecular weight 30-33 kDa. Incubation of the viable cells in isotonic phosphate buffered saline with [32P]NAD results in the incorporation of label into cellular proteins. Dimethyl sulfoxide treatment of the cells downregulates the transferase activity as well as the ADP-ribosylation of cell proteins with extracellular NAD. |
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Authors:
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G Soman; A Haregewoin; R C Hom; R W Finberg |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 176 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1991 Apr |
Date Detail:
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Created Date: 1991-05-20 Completed Date: 1991-05-20 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 301-8 Citation Subset: IM |
Affiliation:
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Laboratory of Infectious Diseases, Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA 02115. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Cell Membrane / enzymology Guanidines / metabolism* Isoenzymes / isolation & purification, metabolism* Kinetics Mice Molecular Weight NAD / metabolism Poly(ADP-ribose) Polymerases / isolation & purification, metabolism* Substrate Specificity |
| Grant Support | |
ID/Acronym/Agency:
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A1 20382//PHS HHS |
| Chemical | |
Reg. No./Substance:
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0/Guanidines; 0/Isoenzymes; 53-84-9/NAD; EC 2.4.2.30/Poly(ADP-ribose) Polymerases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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