| Growth of the yeast Saccharomyces cerevisiae on a non-fermentable substrate: control of energetic yield by the amount of mitochondria. | |
| | |
MedLine Citation:
|
PMID: 10692549 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The purpose of this study was to investigate the long-term control of ATP synthesis during the course of Saccharomyces cerevisiae batch grown on lactate, a purely respiratory substrate. For this, we used a respirometric and on-line calorimetric approach to analyse the energetic balances and the control of energetic metabolism during growth. Enthalpic growth yields assessed by enthalpy balance (taking account of substrate consumption, by-product accumulation, biomass formation and heat dissipation) remained constant during the entire exponential growth. Moreover, at the same time, a parallel decrease in basal respiratory rate and enthalpy flux occurred. It is shown that the decrease in respiration corresponds to a decrease in the amount of mitochondria per cell but not to a change of steady state of oxidative phosphorylation. Taking into account the part of energy used for maintenance, it can be concluded that mitochondria by themselves are the major heat dissipative system in a fully aerobic metabolism, and that the decrease in the amount of mitochondria when growth rate decreases leads to an enthalpic growth yield constant. |
| | |
Authors:
|
L Dejean; B Beauvoit; B Guérin; M Rigoulet |
Related Documents
:
|
15896089 - Interactions of metabolic hormones, adipose tissue and exercise. 7640409 - Cell kinetics of the human mammary lobule during the proliferative and secretory phase ... 16151829 - Effect of training on gh and igf-1 responses to a submaximal exercise in football players. 23516089 - Comparing routine neurorehabilitation program with trunk exercises based on bobath conc... 6361439 - Responses to continuous and intermittent exercise in healthy and insulin-dependent diab... 18334589 - Evidence for acyl-ghrelin modulation of growth hormone release in the fed state. 16249919 - Changes in fluctuation of isometric force following eccentric and concentric exercise o... 7837949 - Indomethacin potentiates exercise-induced reduction in renal hemodynamics in athletes. 10988189 - Descriptors of breathlessness in healthy individuals: distinct and separable constructs. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biochimica et biophysica acta Volume: 1457 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2000 Feb |
Date Detail:
|
Created Date: 2000-06-01 Completed Date: 2000-06-01 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 45-56 Citation Subset: IM |
Affiliation:
|
Institut de Biochimie et Génétique Cellulaires du CNRS, Université Victor Segalen, Bordeaux II, 1 rue Camille Saint Saëns, 33077, Bordeaux, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenosine Triphosphate
/
metabolism Calorimetry Carbonyl Cyanide m-Chlorophenyl Hydrazone Culture Media Energy Metabolism Lactates Mitochondria / enzymology, metabolism* Oxygen Consumption Saccharomyces cerevisiae / growth & development* Uncoupling Agents |
| Chemical | |
Reg. No./Substance:
|
0/Culture Media; 0/Lactates; 0/Uncoupling Agents; 555-60-2/Carbonyl Cyanide m-Chlorophenyl Hydrazone; 56-65-5/Adenosine Triphosphate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Mutations in the tether region of the iron-sulfur protein affect the activity and assembly of the cy...
Next Document: Calcium regulation of oxidative phosphorylation in rat skeletal muscle mitochondria.