| Growth and shape transformations of giant phospholipid vesicles upon interaction with an aqueous oleic acid suspension. | |
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MedLine Citation:
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PMID: 19477312 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The interaction of two types of vesicle systems was investigated: micrometer-sized, giant unilamellar vesicles (GUVs) formed from 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and submicrometer-sized, large unilamellar vesicles (LUVs) formed from oleic acid and oleate, both in a buffered aqueous solution (pH 8.8). Individual POPC GUVs were transferred with a micropipette into a suspension of oleic acid/oleate LUVs, and the shape changes of the GUVs were monitored using optical microscopy. The behavior of POPC GUVs upon transfer into a 0.8mM suspension of oleic acid, in which oleic acid/oleate forms vesicular bilayer structures, was qualitatively different from the behavior upon transfer into a 0.3mM suspension of oleic acid/oleate, in which oleic acid/oleate is predominantly present in the form of monomers and possibly non-vesicular aggregates. In both cases, changes in vesicle morphology were observed within tens of seconds after the transfer. After an initial increase of the vesicle cross-section, the vesicle started to evaginate, spawning dozens of satellite vesicles connected to the mother vesicle with narrow necks or tethers. In 60% of the cases of transfer into a 0.8mM oleic acid suspension, the evagination process reversed and proceeded to the point where the membrane formed invaginations. In some of these cases, several consecutive transitions between invaginated and evaginated shapes were observed. In the remaining 40% of the cases of transfer into the 0.8mM oleic acid suspension and in all cases of vesicle transfer into the 0.3mM oleic acid suspension, no invaginations nor subsequent evaginations were observed. An interpretation of the observed vesicle shape transformation on the basis of the bilayer-couple model is proposed, which takes into account uptake of oleic acid/oleate molecules by the POPC vesicles, oleic acid flip-flop processes and transient pore formation. |
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Authors:
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Primoz Peterlin; Vesna Arrigler; Ksenija Kogej; Sasa Svetina; Peter Walde |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-04-02 |
Journal Detail:
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Title: Chemistry and physics of lipids Volume: 159 ISSN: 1873-2941 ISO Abbreviation: Chem. Phys. Lipids Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-05-29 Completed Date: 2009-07-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0067206 Medline TA: Chem Phys Lipids Country: Ireland |
Other Details:
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Languages: eng Pagination: 67-76 Citation Subset: IM |
Affiliation:
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University of Ljubljana, Faculty of Medicine, Institute of Biophysics, Lipiceva 2, SI-1000 Ljubljana, Slovenia. primoz.peterlin@mf.uni-lj.si |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Fluorescent Dyes
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chemistry Hydrogen-Ion Concentration Oleic Acid / chemistry* Phosphatidylcholines / chemistry Phospholipids / chemistry* Pyrenes / chemistry Spectrometry, Fluorescence Unilamellar Liposomes / chemistry* Water / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Phosphatidylcholines; 0/Phospholipids; 0/Pyrenes; 0/Unilamellar Liposomes; 112-80-1/Oleic Acid; 129-00-0/pyrene; 6753-55-5/1-palmitoyl-2-oleoylphosphatidylcholine; 7732-18-5/Water |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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