Document Detail

Growth restriction and the cerebral metabolic response to acute hypoxia of chick embryos in-ovo: a proton magnetic resonance spectroscopy study.
MedLine Citation:
PMID:  16223529     Owner:  NLM     Status:  MEDLINE    
INTRODUCTION: Perinatal brain injury is more common in growth-restricted (GR) than normally grown (NG) fetuses. This study addresses the hypothesis that chronic oxygen and substrate deprivation during pregnancy will engender an abnormal fetal cerebral metabolic response to acute hypoxia. METHOD: Cerebral metabolite resonance amplitudes relative to that of creatine were measured by proton magnetic resonance spectroscopy in chick embryos on day 19 of incubation. Measurements were obtained before, during and after acute hypoxia (8% ambient oxygen concentration for 44 min) in NG and GR embryos (10% albumen extracted day 0 and 14% oxygen exposure from day 10 of incubation). RESULTS: In both NG and GR embryos, the cerebral lactate/creatine increased during acute hypoxia and slowly recovered after restoration of normoxia. However, the mean (+/-SD) increase in lactate/creatine was significantly less in GR compared to NG embryos (0.51 +/- 0.36 vs. 0.94 +/- 0.36; P = 0.02, t test). Alanine increased during acute hypoxia in NG but not GR embryos. Mean beta-hydroxybutyrate was increased only in GR embryos (0.63 +/- 0.01 vs. 0.22 +/- 0.01; P < 0.001, ANOVA). CONCLUSIONS: Acute hypoxia increases cerebral lactate and alanine in NG chick embryos; these increases are reduced by pre-exposure to substrate deprivation and chronic hypoxia.
James C Dixon; Ernest B Cady; Andrew N Priest; John S Thornton; Donald M Peebles
Related Documents :
16727449 - Splitting of porcine embryos at the four-cell or morula stage.
8322369 - Stage-specific effects of cadmium on preimplantation embryo development and implantatio...
19863469 - Embryonic development and pregnancy test sensitivity: the importance of earlier pregnan...
2714849 - Human embryo associated immunosuppressor factor(s) from pre- and post-implantation stag...
19948839 - Successful transfer of day 10 horse embryos: influence of donor-recipient asynchrony on...
16213839 - Early pronuclear breakdown is a good indicator of embryo quality and viability.
20566259 - Malaria in pregnancy: update on emergency management.
10485309 - Outcome predictors in pregnant opiate and polydrug users.
10670869 - Surgical management of biliary gallstone disease during pregnancy.
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-10-11
Journal Detail:
Title:  Brain research. Developmental brain research     Volume:  160     ISSN:  0165-3806     ISO Abbreviation:  Brain Res. Dev. Brain Res.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-02     Completed Date:  2006-02-07     Revised Date:  2007-08-13    
Medline Journal Info:
Nlm Unique ID:  8908639     Medline TA:  Brain Res Dev Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  203-10     Citation Subset:  IM    
Department of Obstetrics and Gynaecology, University College London, 86-96 Chenies Mews, London WC1E 6HX, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
3-Hydroxybutyric Acid / metabolism
Alanine / metabolism
Anoxia / metabolism*
Aspartic Acid / analogs & derivatives,  metabolism
Cerebellum / embryology,  metabolism*
Chick Embryo
Fetal Hypoxia / metabolism*
Lactic Acid / metabolism
Magnetic Resonance Spectroscopy / methods*
Restraint, Physical / methods
Time Factors
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/Protons; 300-85-6/3-Hydroxybutyric Acid; 50-21-5/Lactic Acid; 56-41-7/Alanine; 56-84-8/Aspartic Acid; 997-55-7/N-acetylaspartate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Chronic repetitive transcranial magnetic stimulation is antidepressant but not anxiolytic in rat mod...
Next Document:  Alterations in cell-mediated immune response in subacute sclerosing panencephalitis.