Document Detail


Growth restriction and the cerebral metabolic response to acute hypoxia of chick embryos in-ovo: a proton magnetic resonance spectroscopy study.
MedLine Citation:
PMID:  16223529     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: Perinatal brain injury is more common in growth-restricted (GR) than normally grown (NG) fetuses. This study addresses the hypothesis that chronic oxygen and substrate deprivation during pregnancy will engender an abnormal fetal cerebral metabolic response to acute hypoxia. METHOD: Cerebral metabolite resonance amplitudes relative to that of creatine were measured by proton magnetic resonance spectroscopy in chick embryos on day 19 of incubation. Measurements were obtained before, during and after acute hypoxia (8% ambient oxygen concentration for 44 min) in NG and GR embryos (10% albumen extracted day 0 and 14% oxygen exposure from day 10 of incubation). RESULTS: In both NG and GR embryos, the cerebral lactate/creatine increased during acute hypoxia and slowly recovered after restoration of normoxia. However, the mean (+/-SD) increase in lactate/creatine was significantly less in GR compared to NG embryos (0.51 +/- 0.36 vs. 0.94 +/- 0.36; P = 0.02, t test). Alanine increased during acute hypoxia in NG but not GR embryos. Mean beta-hydroxybutyrate was increased only in GR embryos (0.63 +/- 0.01 vs. 0.22 +/- 0.01; P < 0.001, ANOVA). CONCLUSIONS: Acute hypoxia increases cerebral lactate and alanine in NG chick embryos; these increases are reduced by pre-exposure to substrate deprivation and chronic hypoxia.
Authors:
James C Dixon; Ernest B Cady; Andrew N Priest; John S Thornton; Donald M Peebles
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-10-11
Journal Detail:
Title:  Brain research. Developmental brain research     Volume:  160     ISSN:  0165-3806     ISO Abbreviation:  Brain Res. Dev. Brain Res.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-02     Completed Date:  2006-02-07     Revised Date:  2007-08-13    
Medline Journal Info:
Nlm Unique ID:  8908639     Medline TA:  Brain Res Dev Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  203-10     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynaecology, University College London, 86-96 Chenies Mews, London WC1E 6HX, UK.
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MeSH Terms
Descriptor/Qualifier:
3-Hydroxybutyric Acid / metabolism
Alanine / metabolism
Animals
Anoxia / metabolism*
Aspartic Acid / analogs & derivatives,  metabolism
Cerebellum / embryology,  metabolism*
Chick Embryo
Fetal Hypoxia / metabolism*
Lactic Acid / metabolism
Magnetic Resonance Spectroscopy / methods*
Protons*
Restraint, Physical / methods
Time Factors
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/Protons; 300-85-6/3-Hydroxybutyric Acid; 50-21-5/Lactic Acid; 56-41-7/Alanine; 56-84-8/Aspartic Acid; 997-55-7/N-acetylaspartate

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