Document Detail


Growth requirements of human mammary epithelial cells in culture.
MedLine Citation:
PMID:  304048     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Colony-forming epithelial cells can be separated from the non-dividing "foam cells" in human milk by differential adhesion to glass and freezing. The growth of such partially purified mammary epithelial cells is stimulated by co-culture with non-dividing feeder cells. Foam cells, mitomycin-treated mouse fibroblast lines and human mammary fibroblasts and calf lens epithelial cells are all effective in promoting mammary epithelial cell growth. Contact between epithelial cells and feeders is not required for the growth-promoting effect. The mitogenic effect of epidermal growth factor on mammary epithelial cells also requires feeder cell activity.
Authors:
J Taylor-Papadimitriou; M Shearer; M G Stoker
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  20     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  1977 Dec 
Date Detail:
Created Date:  1978-02-18     Completed Date:  1978-02-18     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  903-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoradiography
Breast / cytology*
Cattle
Cell Division / drug effects
Cell Separation
Cells, Cultured
Epidermal Growth Factor / pharmacology
Epithelial Cells
Female
Fibroblasts / cytology
Freezing
Humans
Lens, Crystalline / cytology
Milk, Human / cytology*
Mitomycins / pharmacology
Chemical
Reg. No./Substance:
0/Mitomycins; 62229-50-9/Epidermal Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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