| Growth requirements of human mammary epithelial cells in culture. | |
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MedLine Citation:
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PMID: 304048 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Colony-forming epithelial cells can be separated from the non-dividing "foam cells" in human milk by differential adhesion to glass and freezing. The growth of such partially purified mammary epithelial cells is stimulated by co-culture with non-dividing feeder cells. Foam cells, mitomycin-treated mouse fibroblast lines and human mammary fibroblasts and calf lens epithelial cells are all effective in promoting mammary epithelial cell growth. Contact between epithelial cells and feeders is not required for the growth-promoting effect. The mitogenic effect of epidermal growth factor on mammary epithelial cells also requires feeder cell activity. |
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Authors:
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J Taylor-Papadimitriou; M Shearer; M G Stoker |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: International journal of cancer. Journal international du cancer Volume: 20 ISSN: 0020-7136 ISO Abbreviation: Int. J. Cancer Publication Date: 1977 Dec |
Date Detail:
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Created Date: 1978-02-18 Completed Date: 1978-02-18 Revised Date: 2007-07-24 |
Medline Journal Info:
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Nlm Unique ID: 0042124 Medline TA: Int J Cancer Country: DENMARK |
Other Details:
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Languages: eng Pagination: 903-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Autoradiography Breast / cytology* Cattle Cell Division / drug effects Cell Separation Cells, Cultured Epidermal Growth Factor / pharmacology Epithelial Cells Female Fibroblasts / cytology Freezing Humans Lens, Crystalline / cytology Milk, Human / cytology* Mitomycins / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Mitomycins; 62229-50-9/Epidermal Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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