Document Detail


Growth requirements of Haemophilus somnus.
MedLine Citation:
PMID:  7153333     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The growth factor needs of Haemophilus somnus, which have not been defined to date, were found to be provided by 1% IsoVitaleX (IVX; BBL Microbiology Systems) in tryptose broth. Some growth, however, occurred in unsupplemented tryptose broth. Of the ingredients of IVX, cocarboxylase was found to stimulate growth to about the same degree as the total supplement. Cocarboxylase was without direct effect in 2% peptone broth, which supported no growth of 25 H. somnus strains until supplemented with IVX, optimally at the 10% level. This could be substituted for by proportional amounts of cysteine or cystine, but by no other IVX ingredient. Cysteine-cystine and IVX but not cocarboxylase supplementation allowed H. somnus to grow in Eagle minimal medium, a completely synthetic medium, but attempts at serial passage were unsuccessful.
Authors:
M Merino; E L Biberstein
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  16     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1982 Nov 
Date Detail:
Created Date:  1983-03-11     Completed Date:  1983-03-11     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  798-802     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Culture Media*
Cysteine / pharmacology
Cystine / pharmacology
Haemophilus / growth & development*
Chemical
Reg. No./Substance:
0/Culture Media; 52-90-4/Cysteine; 56-89-3/Cystine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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