| Growth regulation of a human mature B cell line, B104, by anti-IgM and anti-IgD antibodies. | |
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MedLine Citation:
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PMID: 1988498 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An EBNA- human B lymphoma cell line, B104, was established. B104 cells express IgD as well as IgM on their surface, which is thought to be a basic characteristic of mature B cells. The growth of B104 cells was inhibited by treatment with a panel of anti-IgM antibodies. Cell cycle analyses revealed that the transition of B104 cells from the G2/M to the G0/G1 phase of the cell cycle was markedly inhibited by treatment with anti-IgM antibodies. Progression of B104 cells to the M phase of the cell cycle was found to be suppressed in the presence of anti-IgM antibodies. In contrast, both the entrance of G0/G1 phase cells into the S phase and the progression of S phase cells to the G2/M phase of the cell cycle did not seem to be inhibited significantly by treatment with anti-IgM antibodies. These results indicate that the mechanism of the inhibition of growth of B104 cells by anti-IgM antibodies is blockage of the transition from the G2 to the M phase of the cell cycle. In contrast to anti-IgM antibodies, anti-IgD antibodies could not cause growth inhibition of B104 cells at all. B cell growth factors such as IL-4 and IL-6 had no effect on the inhibition of growth of B104 cells by anti-IgM antibody. IFN-alpha and -beta, which have no B cell growth factor activity, did increase the number of cells that survived the treatment with anti-IgM antibodies. B104 is an excellent experimental model for the study of the mechanism of signal transduction through sIg as well as the functional difference between sIgM and sIgD. |
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Authors:
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K M Kim; T Yoshimura; H Watanabe; T Ishigami; M Nambu; D Hata; Y Higaki; M Sasaki; T Tsutsui; M Mayumi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 146 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 1991 Feb |
Date Detail:
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Created Date: 1991-02-25 Completed Date: 1991-02-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 819-25 Citation Subset: AIM; IM |
Affiliation:
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Department of Pediatrics, Faculty of Medicine, Kyoto University, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Anti-Idiotypic
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immunology* Antigens, Surface / analysis Antigens, Viral / analysis B-Lymphocytes / immunology* Calcium / analysis Cell Cycle Cytotoxicity, Immunologic DNA / biosynthesis Epstein-Barr Virus Nuclear Antigens Humans Immunoglobulin D / immunology* Immunoglobulin M / immunology* Interferon Type I / pharmacology Lymphoma, B-Cell / genetics, immunology*, pathology Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Anti-Idiotypic; 0/Antigens, Surface; 0/Antigens, Viral; 0/Epstein-Barr Virus Nuclear Antigens; 0/Immunoglobulin D; 0/Immunoglobulin M; 0/Interferon Type I; 7440-70-2/Calcium; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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