Document Detail


Growth properties of a human melanoma cell line are altered by adeno-associated parvovirus type 2.
MedLine Citation:
PMID:  7829227     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The adeno-associated parvoviruses (AAV) are known for their oncosuppressive activity. This report shows that infection of the human melanoma cell line MKr with AAV-2 leads to chromosomal integration of the viral DNA. The resulting cultures show decrease of saturation density and reduction in their ability to form colonies from single cells and thus to resemble normal cells. After single-cell cloning they may give rise to growth-arrested, terminally differentiating large, multinucleated cells with dendrite-like extensions, large nucleoli and the ability to synthesize melanin pigment. In cultured melanoma cells, melanin synthesis is correlated with cellular senescence, and since senescent cells cease to proliferate and withdraw from the cell cycle, the apparent induction of growth arrest and terminal differentiation in malignant cells by AAV may provide one explanation for the anti-neoplastic activity of these viruses.
Authors:
U Bantel-Schaal
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  60     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  1995 Jan 
Date Detail:
Created Date:  1995-02-17     Completed Date:  1995-02-17     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  269-74     Citation Subset:  IM    
Affiliation:
Forschungsschwerpunkt Angewandte Tumorvirologie 0625, Deutsches Krebsforschungszentrum Heidelberg, Germany.
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MeSH Terms
Descriptor/Qualifier:
DNA, Viral / analysis
Dependovirus / genetics,  physiology*
Humans
Melanoma / pathology*,  virology
Parvovirus / genetics,  physiology*
Tumor Cells, Cultured
Virus Integration
Chemical
Reg. No./Substance:
0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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