Document Detail


Growth phenotype screening of Schizosaccharomyces pombe using a Lensless microscope.
MedLine Citation:
PMID:  24291754     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The Lensless microscope has a large field of view and allows the capture of the diffraction pattern from a large number of cells simultaneously. A simple algorithm to measure intensity changes in the Airy Disc First Fringe (ADFF) has been derived to follow the growth characteristics of the unicellular yeast Schizosaccharomyces pombe. The performance of the algorithm is calibrated using comparison between optical image and ADFF analysis of polystyrene microspheres with known dimensions and has an accuracy of 5% over all lengths above the diffraction-limited measurements. We have observed the growth characteristics of S. pombe for N=100 cells to determine the growth phenotype distributions of Length (L(t=0)) and width (W(t=0)) on arrival at the surface, lag phase adjustment to the new growth conditions (B), the length at birth, LB, and cell cycle length, tcell. The observed cell width distribution has a median width of 3.9 (±0.1)µm, as expected, but a non-normal distribution. Similarly, all growth parameters studied, L(t=0), LB and cell cycle time are phenotypes with non-normal distributions but with medians consistent with the literature values.
Authors:
Lynsey A Penwill; Gwendoline E Batten; Stefania Castegnetti; Andrew M Shaw
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-11-13
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  54C     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2013 Nov 
Date Detail:
Created Date:  2013-12-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  -    
Other Details:
Languages:  ENG     Pagination:  345-350     Citation Subset:  -    
Copyright Information:
© 2013 Published by Elsevier B.V.
Affiliation:
Biosciences, College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter, UK.
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