Document Detail


Growth patterns and nuclear distribution in white muscle fibers from black sea bass, Centropristis striata: evidence for the influence of diffusion.
MedLine Citation:
PMID:  21430198     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study investigated the influence of fiber size on the distribution of nuclei and fiber growth patterns in white muscle of black sea bass, Centropristis striata, ranging in body mass from 0.45 to 4840 g. Nuclei were counted in 1 μm optical sections using confocal microscopy of DAPIand Acridine-Orange-stained muscle fibers. Mean fiber diameter increased from 36±0.87 μm in the 0.45 g fish to 280±5.47 μm in the 1885 g fish. Growth beyond 2000 g triggered the recruitment of smaller fibers, thus significantly reducing mean fiber diameter. Nuclei in the smaller fibers were exclusively subsarcolemmal (SS), whereas in larger fibers nuclei were more numerous and included intermyofibrillar (IM) nuclei. There was a significant effect of body mass on nuclear domain size (F=118.71, d.f.=3, P<0.0001), which increased to a maximum in fish of medium size (282-1885 g) and then decreased in large fish (>2000 g). Although an increase in the number of nuclei during fiber growth can help preserve the myonuclear domain, the appearance of IM nuclei during hypertrophic growth seems to be aimed at maintaining short effective diffusion distances for nuclear substrates and products. If only SS nuclei were present throughout growth, the diffusion distance would increase in proportion to the radius of the fibers. These observations are consistent with the hypothesis that changes in nuclear distribution and fiber growth patterns are mechanisms for avoiding diffusion limitation during animal growth.
Authors:
Carolina Priester; Lindsay C Morton; Stephen T Kinsey; Wade O Watanabe; Richard M Dillaman
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of experimental biology     Volume:  214     ISSN:  1477-9145     ISO Abbreviation:  J. Exp. Biol.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-24     Completed Date:  2011-07-14     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  0243705     Medline TA:  J Exp Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1230-9     Citation Subset:  IM    
Affiliation:
Department of Biology and Marine Biology, University of North Carolina Wilmington, 601 South College Road, Wilmington, NC 28403, USA. cpl3791@uncw.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Bass / anatomy & histology*,  growth & development*
Cell Nucleus / metabolism*
Diffusion
Muscle Fibers, Fast-Twitch / cytology*,  physiology*
Grant Support
ID/Acronym/Agency:
R15-AR-052708/AR/NIAMS NIH HHS
Comments/Corrections

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