Document Detail


Growth pattern of the human promyelocytic leukaemia cell line HL60.
MedLine Citation:
PMID:  6955024     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to characterize the growth pattern of the human promyelocytic leukaemia cell line HL60, its kinetic parameters were studied. The doubling time was calculated from serial cell counts, the duration of the various cell cycle phases from the analysis of the labelled mitoses curve, and quiescent population from continuous labelling experiments. Proliferation in culture was exponential up to a saturation density of about 3.0 x 10(6) cells/ml, with a doubling time of 34.0 hr. The cell cycle duration was 24.3 +/- 4.1 hr (SD), and that of the cell cycle phases was: G1, 3.8 +/- 2.2 hr; S, 15.1 +/- 3 hr; and G2, 4 +/0 1.2 hr. The growth fraction was 0.85, and cell loss was restricted to the quiescent cells. The HL60 cell line, with fully characterized kinetics, provides a useful tool for the vitro study of substances which may affect human leukaemic myelopoietic proliferation.
Authors:
P Foa; A T Maiolo; L Lombardi; H Toivonen; T Rytömaa; E E Polli
Related Documents :
7883794 - Regulation of the cell cycle timing of start in fission yeast by the rum1+ gene.
22534074 - Jararhagin, a hemorrhagic snake venom metalloproteinase from bothrops jararaca.
10937344 - Synergistic antiproliferative effect of delta 12-prostaglandin j2 (delta 12-pgj2) and h...
22353164 - Nucleotides released from aβ₁₋₄₂ -treated microglial cells increase cell migration and ...
24350594 - Plasticity of in vitro-generated urothelial cells for functional tissue formation.
21927514 - Live staining and isolation of specific hormone-producing cells from rat anterior pitui...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell and tissue kinetics     Volume:  15     ISSN:  0008-8730     ISO Abbreviation:  Cell Tissue Kinet     Publication Date:  1982 Jul 
Date Detail:
Created Date:  1982-10-29     Completed Date:  1982-10-29     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0174107     Medline TA:  Cell Tissue Kinet     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  399-404     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle
Cell Division
Cell Line
Humans
Leukemia, Experimental / pathology*
Leukemia, Myeloid, Acute / pathology*
Mathematics
Mitosis

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Phase I--II evaluation of a new anthracycline antibiotic, aclacinomycin A, in adults with refractory...
Next Document:  Membrane insertion and oligomeric assembly of HLA-DR histocompatibility antigens.