Document Detail

Growth pattern of the human promyelocytic leukaemia cell line HL60.
MedLine Citation:
PMID:  6955024     Owner:  NLM     Status:  MEDLINE    
In order to characterize the growth pattern of the human promyelocytic leukaemia cell line HL60, its kinetic parameters were studied. The doubling time was calculated from serial cell counts, the duration of the various cell cycle phases from the analysis of the labelled mitoses curve, and quiescent population from continuous labelling experiments. Proliferation in culture was exponential up to a saturation density of about 3.0 x 10(6) cells/ml, with a doubling time of 34.0 hr. The cell cycle duration was 24.3 +/- 4.1 hr (SD), and that of the cell cycle phases was: G1, 3.8 +/- 2.2 hr; S, 15.1 +/- 3 hr; and G2, 4 +/0 1.2 hr. The growth fraction was 0.85, and cell loss was restricted to the quiescent cells. The HL60 cell line, with fully characterized kinetics, provides a useful tool for the vitro study of substances which may affect human leukaemic myelopoietic proliferation.
P Foa; A T Maiolo; L Lombardi; H Toivonen; T Rytömaa; E E Polli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell and tissue kinetics     Volume:  15     ISSN:  0008-8730     ISO Abbreviation:  Cell Tissue Kinet     Publication Date:  1982 Jul 
Date Detail:
Created Date:  1982-10-29     Completed Date:  1982-10-29     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0174107     Medline TA:  Cell Tissue Kinet     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  399-404     Citation Subset:  IM    
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MeSH Terms
Cell Cycle
Cell Division
Cell Line
Leukemia, Experimental / pathology*
Leukemia, Myeloid, Acute / pathology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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