Document Detail


Growth of normal human ovarian surface epithelial cells in reduced-serum and serum-free media.
MedLine Citation:
PMID:  7680339     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The human ovarian surface epithelium (OSE) is believed responsible for over 85% of ovarian cancers, yet little is known about the normal biology of these cells. To date, culture of OSE has only been reported in media with high serum supplements. We have developed two media, one with less than 1% of serum (OSEM-1) and the other comprised of highly purified and defined materials (OSEM-2), which allow us to study OSE under relatively defined conditions. By substituting 0.05% of Pedersen's fetuin for 15% fetal bovine serum (FBS) with Medium 199/MCDB105 basal medium, the cell numbers reached 50 to 60% of those in the presence of 15% FBS over 7 days. However, over several weeks, the total number of population doublings achieved were comparable to those in 15% FBS. Addition of insulin, transferrin, ethanolamine, lipoic acid, and phosphatidylcholine to the medium with Pedersen's fetuin (OSEM-1) enhanced growth up to 20% more than in their absence. Supplementation of M199/105 with highly purified (> 99%) fetuin, alpha 2-macroglobulin, and hydrocortisone resulted in a defined medium (OSEM-2) that permitted 1 to 2 doublings/7 days. In addition, cells maintained a more normal, epithelial-like morphology in culture for a longer period in the presence of Pedersen's or purified fetuin than in M199/105/15% FBS, thus increasing the number of morphologically normal cells available for experimentation. Addition of 0.05% Pedersen's fetuin to M199/105 in the presence of 6 to 8% FBS resulted in levels of growth equivalent to those in M199/105/15% FBS alone. We are now able to study the effects of various compounds on the growth and differentiation of OSE under defined conditions, and have reduced the requirement for FBS to produce large numbers of OSE cells.
Authors:
W M Elliott; N Auersperg
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  In vitro cellular & developmental biology : journal of the Tissue Culture Association     Volume:  29A     ISSN:  0883-8364     ISO Abbreviation:  In Vitro Cell. Dev. Biol.     Publication Date:  1993 Jan 
Date Detail:
Created Date:  1993-04-06     Completed Date:  1993-04-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8506951     Medline TA:  In Vitro Cell Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  9-18     Citation Subset:  IM    
Affiliation:
Department of Anatomy, University of British Columbia, Vancouver, Canada.
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MeSH Terms
Descriptor/Qualifier:
Cell Count
Cell Differentiation / drug effects
Cell Division / drug effects*
Cells, Cultured / drug effects
Culture Media / pharmacology
Culture Media, Serum-Free / pharmacology*
Epithelial Cells
Female
Humans
Keratins / analysis
Ovary / cytology
alpha-Fetoproteins / pharmacology
Chemical
Reg. No./Substance:
0/Culture Media; 0/Culture Media, Serum-Free; 0/alpha-Fetoproteins; 68238-35-7/Keratins

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