Document Detail


Growth kinetics of gel-immobilized yeast cells studied by on-line microscopy.
MedLine Citation:
PMID:  7763717     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A 'microscope reactor' was used to study on-line the dynamics of gel immobilized cell systems. The applicability of the reactor is demonstrated by a study of the growth kinetics of Saccharomyces cerevisiae entrapped in 2% calcium alginate. The specific growth rates of single immobilized cells and free cells were measured. The growth of a microcolony in Ca-alginate was followed and the specific growth rate of the cells in the microcolony determined. A simple growth model was used to estimate the cell volume fraction of the yeast cells in the microcolony. As internal and external mass transfer limitations can be neglected and immobilized cell growth rates were found to be identical to those of free cells, one may conclude that immobilization does not influence cell growth under our experimental conditions.
Authors:
R Willaert; G Baron
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  39     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  1993 Jun 
Date Detail:
Created Date:  1993-07-30     Completed Date:  1993-07-30     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  347-52     Citation Subset:  B    
Affiliation:
Department of Chemical Engineering, Vrije Universiteit Brussel, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Alginates
Biotechnology
Cell Division
Gels
Glucuronic Acid
Hexuronic Acids
Kinetics
Microscopy / instrumentation
Models, Biological
Saccharomyces cerevisiae / cytology,  growth & development*
Chemical
Reg. No./Substance:
0/Alginates; 0/Gels; 0/Hexuronic Acids; 576-37-4/Glucuronic Acid; 9005-32-7/alginic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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