Document Detail


Growth inhibitory and stimulatory effects of retinoic acid on murine 3T3 cells.
MedLine Citation:
PMID:  6951195     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
All-trans-beta-retinoic acid (RA) has both comitogenic and antiproliferative effects on murine Swiss 3T3 cells. Treatment of quiescent 3T3 cells for less than 24 hr with micromolar concentrations of RA potentiates subsequent mitogenic response of those cells to phorbol 12-myristate 13-acetate. Longer exposures of 3T3 cells to RA result in inhibition of DNA replication as measured by [3H]thymidine incorporation and decreased growth rates and saturation densities for cells grown in either 2% or 10% serum. Both the comitogenic and antiproliferative activities of RA for 3T3 cells are RA-dose dependent. RA-induced decreases in the 3T3 cell saturation density are reversible only after resuspension of cells by trypsinization and replating. Treatment of 3T3 cells for 48 hr with RA inhibits the rate of [3H]thymidine incorporation by 35--50%, while autoradiographic data show that labeling indices are similar to control values. Equal percentages of control and 48-hr RA-treated quiescent 3T3 cells respond to subsequent stimulation with 10% serum as determined by autoradiographic and flow cytometric analyses. However, the progression of RA-treated cells through the S phase of the cell cycle is slowed. These data suggest that inhibition of 3T3 cell proliferation by RA is established after a minimum 24-hr treatment and that this inhibition is the result of a decreased rate of DNA replication in S-phase cells.
Authors:
E W Schroder; E Rapaport; A K Kabcenell; P H Black
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  79     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1982 Mar 
Date Detail:
Created Date:  1982-06-21     Completed Date:  1982-06-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1549-52     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division / drug effects*
Cell Line
DNA / biosynthesis
DNA Replication / drug effects
Flow Cytometry
Mice
Mitosis / drug effects
Tretinoin / pharmacology*
Grant Support
ID/Acronym/Agency:
CA 28107/CA/NCI NIH HHS; CA 28803/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
302-79-4/Tretinoin; 9007-49-2/DNA
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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