Document Detail


Growth inhibitory effects of casein hydrolysates on human cancer cell lines.
MedLine Citation:
PMID:  20053315     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to investigate the effects of unhydrolysed/intact casein and eight different sodium casein hydrolysates (a-h) on the viability and growth of human cancer cell lines. Both human Jurkat T cells and Caco-2 cells were incubated with increasing concentrations of the test compounds (0.5-10% v/v) for 24 h. Cell viability was assessed using the MTT, lactate dehydrogenase (LDH) release and Trypan Blue assays. Cell growth was monitored using the MTT, Trypan Blue and Bromodeoxyuridine (BrdU) proliferation assays. Casein hydrolysates b, c and f had an inhibitory effect on the viability and growth of both cell lines. The casein hydrolysates did not negatively affect the membrane integrity of both Jurkat and Caco-2 cells. In Jurkat cells hydrolysates a and h had an inhibitory effect on DNA synthesis after 24 h, while in Caco-2 cells DNA synthesis was not affected. In conclusion, we found that the different casein hydrolysates had cell-specific effects which target particular functions within the cell. Overall, casein hydrolysates had no effect on membrane integrity while they had varied effects on mitochondrial activity and DNA synthesis in the different cell lines.
Authors:
Martha Phelan; S Aisling Aherne; Dara O'Sullivan; Richard J FitzGerald; Nora M O'Brien
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-07
Journal Detail:
Title:  The Journal of dairy research     Volume:  77     ISSN:  1469-7629     ISO Abbreviation:  J. Dairy Res.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-12     Completed Date:  2010-07-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985125R     Medline TA:  J Dairy Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  176-82     Citation Subset:  IM    
Affiliation:
Department of Food and Nutritional Sciences, University College Cork, Cork, Ireland.
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MeSH Terms
Descriptor/Qualifier:
Caco-2 Cells
Caseins / pharmacology
Cell Membrane / drug effects,  metabolism
Cell Proliferation / drug effects*
Cell Survival / drug effects*
DNA Replication / drug effects
Down-Regulation
Humans
Jurkat Cells
Mitochondria / drug effects,  metabolism
Neoplasms / drug therapy,  metabolism,  pathology
Organ Specificity
Chemical
Reg. No./Substance:
0/Caseins; 65072-00-6/casein hydrolysate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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