| Growth inhibitory effects of casein hydrolysates on human cancer cell lines. | |
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MedLine Citation:
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PMID: 20053315 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to investigate the effects of unhydrolysed/intact casein and eight different sodium casein hydrolysates (a-h) on the viability and growth of human cancer cell lines. Both human Jurkat T cells and Caco-2 cells were incubated with increasing concentrations of the test compounds (0.5-10% v/v) for 24 h. Cell viability was assessed using the MTT, lactate dehydrogenase (LDH) release and Trypan Blue assays. Cell growth was monitored using the MTT, Trypan Blue and Bromodeoxyuridine (BrdU) proliferation assays. Casein hydrolysates b, c and f had an inhibitory effect on the viability and growth of both cell lines. The casein hydrolysates did not negatively affect the membrane integrity of both Jurkat and Caco-2 cells. In Jurkat cells hydrolysates a and h had an inhibitory effect on DNA synthesis after 24 h, while in Caco-2 cells DNA synthesis was not affected. In conclusion, we found that the different casein hydrolysates had cell-specific effects which target particular functions within the cell. Overall, casein hydrolysates had no effect on membrane integrity while they had varied effects on mitochondrial activity and DNA synthesis in the different cell lines. |
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Authors:
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Martha Phelan; S Aisling Aherne; Dara O'Sullivan; Richard J FitzGerald; Nora M O'Brien |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-01-07 |
Journal Detail:
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Title: The Journal of dairy research Volume: 77 ISSN: 1469-7629 ISO Abbreviation: J. Dairy Res. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-12 Completed Date: 2010-07-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985125R Medline TA: J Dairy Res Country: England |
Other Details:
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Languages: eng Pagination: 176-82 Citation Subset: IM |
Affiliation:
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Department of Food and Nutritional Sciences, University College Cork, Cork, Ireland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Caco-2 Cells Caseins / pharmacology Cell Membrane / drug effects, metabolism Cell Proliferation / drug effects* Cell Survival / drug effects* DNA Replication / drug effects Down-Regulation Humans Jurkat Cells Mitochondria / drug effects, metabolism Neoplasms / drug therapy, metabolism, pathology Organ Specificity |
| Chemical | |
Reg. No./Substance:
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0/Caseins; 65072-00-6/casein hydrolysate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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