Document Detail


Growth inhibition of newly established human glioma cell lines by leukemia inhibitory factor.
MedLine Citation:
PMID:  9760066     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have established three new cell lines deriving from malignant human gliomas. The cell lines were described in terms of both morphology and growth characteristics. Most cells in all three cell lines expressed the neuroepithelial marker protein GFAP. In terms of growth characteristics, the cells showed only slight differences. The cell lines showed no expression of the neural form of the c-src gene, pp60c-srcN, but did express the ubiquitous form, pp60c-src. The established glioma cell lines were also examined for expression of members of the neuropoietic cytokine family, CNTF and LIF, and their respective receptor components CNTFRalpha, LIFRbeta and gp130. With the exception of CNTFRalpha both the ligands and their receptor components were expressed in similar amounts in all three cell lines. The presence of ligand and receptor prompted us to study the effects of exogenously supplied factors on the growth of the glioma cell lines. Whereas LIF induced a high c-fos expression, only low c-fos induction was observed upon CNTF treatment. Accordingly, CNTF did not have any noticeable effects on glioma cell growth in culture, while LIF mediated an inhibiting effect on the growth of the three glioma cell lines in culture.
Authors:
H Halfter; J Kremerskothen; J Weber; U Hacker-Klom; A Barnekow; E B Ringelstein; F Stögbauer
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neuro-oncology     Volume:  39     ISSN:  0167-594X     ISO Abbreviation:  J. Neurooncol.     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1998-12-30     Completed Date:  1998-12-30     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8309335     Medline TA:  J Neurooncol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  1-18     Citation Subset:  IM    
Affiliation:
Klinik für Neurologie, Westfälische Wilhelms-Universität Münster, Germany.
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MeSH Terms
Descriptor/Qualifier:
Aneuploidy
Animals
Antigens, CD / biosynthesis,  genetics
Brain Neoplasms / chemistry,  pathology*
Cell Division
Chromosome Aberrations
Ciliary Neurotrophic Factor
Cytokine Receptor gp130
Gene Expression Regulation, Neoplastic
Genes, fos
Glial Fibrillary Acidic Protein / biosynthesis,  genetics
Glioma / genetics,  metabolism,  pathology*
Growth Inhibitors / biosynthesis,  genetics,  pharmacology*
Humans
Interleukin-6*
Karyotyping
Leukemia Inhibitory Factor
Leukemia Inhibitory Factor Receptor alpha Subunit
Lymphokines / biosynthesis,  genetics,  pharmacology*
Membrane Glycoproteins / biosynthesis,  genetics
Mice
Mice, Nude
Neoplasm Proteins / biosynthesis,  genetics
Neoplasm Transplantation
Nerve Tissue Proteins / biosynthesis,  genetics
Proto-Oncogene Proteins c-fos / biosynthesis
Proto-Oncogene Proteins pp60(c-src) / biosynthesis,  genetics
Receptor Protein-Tyrosine Kinases / biosynthesis,  genetics
Receptor, Ciliary Neurotrophic Factor
Receptors, Cytokine / biosynthesis,  genetics
Receptors, Nerve Growth Factor / biosynthesis,  genetics
Receptors, OSM-LIF
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Ciliary Neurotrophic Factor; 0/Glial Fibrillary Acidic Protein; 0/Growth Inhibitors; 0/IL6ST protein, human; 0/Il6st protein, mouse; 0/Interleukin-6; 0/LIF protein, human; 0/LIFR protein, human; 0/Leukemia Inhibitory Factor; 0/Leukemia Inhibitory Factor Receptor alpha Subunit; 0/Lif protein, mouse; 0/Lifr protein, mouse; 0/Lymphokines; 0/Membrane Glycoproteins; 0/Neoplasm Proteins; 0/Nerve Tissue Proteins; 0/Proto-Oncogene Proteins c-fos; 0/Receptor, Ciliary Neurotrophic Factor; 0/Receptors, Cytokine; 0/Receptors, Nerve Growth Factor; 0/Receptors, OSM-LIF; 133483-10-0/Cytokine Receptor gp130; EC 2.7.10.1/Receptor Protein-Tyrosine Kinases; EC 2.7.10.2/Proto-Oncogene Proteins pp60(c-src)

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